In this study, we investigated the immune-enhancing activity of water extracts from Hydrangea macrophylla subsp. serrata (WE-HML). WE-HML increased cell viability and production of immunomodulators, which contributed to activating phagocytic activity in RAW264.7 cells. Inhibition of JNK and NF-κB reduced the production of immunomodulators by WE-HML. ROS inhibition suppressed the production of immunomodulators, and the activation of JNK and NF-κB signaling by WE-HML. TLR4 inhibition attenuated the production of immunomodulators, and activation of JNK and NF-κB signaling by WE-HML. In the immunosuppressed mouse model, WE-HML increased the spleen index, the levels of the cytokines, the numbers of white blood cells, lymphocytes, and neutrophils. However, WE-HML inhibited LPS-mediated overproduction of pro-inflammatory mediators in RAW264.7 cells, which indicated that WE-HML may have anti-inflammatory activity under excessive inflammatory conditions. Taken together, WE-HML may be considered to have immune-enhancing activity and expected to be used as a potential immune-enhancing agent.

在这项研究中，我们调查了绣球花亚种的水提取物的免疫增强活性。锯齿状（WE-HML）。WE-HML增加了细胞活力和免疫调节剂的产生，这有助于激活RAW264.7细胞的吞噬活性。抑制JNK和NF-κB减少了WE-HML产生的免疫调节剂。ROS抑制抑制了WE-HML免疫调节剂的产生以及JNK和NF-κB信号的激活。TLR4抑制减弱了WE-HML的免疫调节剂的产生以及JNK和NF-κB信号的激活。在免疫抑制的小鼠模型中，WE-HML可提高脾脏指数，细胞因子水平，白细胞，淋巴细胞和中性粒细胞的数量。然而，WE-HML抑制了RAW264.7细胞中LPS介导的促炎性介质的过度产生，这表明WE-HML在过度的炎症条件下可能具有抗炎活性。在一起