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Preharvest application of methyl salicylate, acetyl salicylic acid and salicylic acid alleviated disease caused by Botrytis cinerea through stimulation of antioxidant system in table grapes.
International Journal of Food Microbiology ( IF 5.4 ) Pub Date : 2020-07-31 , DOI: 10.1016/j.ijfoodmicro.2020.108807
María Emma García-Pastor 1 , María José Giménez 1 , Pedro J Zapata 1 , Fabián Guillén 1 , Juan Miguel Valverde 1 , María Serrano 2 , Daniel Valero 1
Affiliation  

The main goal of this study was to describe impact of preharvest application of methyl salicylate (MeSA), acetyl salicylic acid (ASA) and salicylic acid (SA) on the reduction of disease caused by Botrytis cinerea in two table grape cultivars (‘Crimson’ and ‘Magenta’). Based on previous studies, MeSA and SA were applied at 0.1 and 0.01 mM for both cultivars, while ASA was applied at 1 mM in ‘Crimson’ and 0.1 mM in ‘Magenta’. At time of harvest, berry maturity-quality attributes, bioactive compounds and antioxidant enzymes were determined. In addition, grapes were artificially inoculated with B. cinerea spores, and the berries were ranked for visual decay incidence after 5 days of inoculation. Salicylates preharvest treatments led to higher total acidity, content of bioactive compounds and activity of antioxidant enzymes in treated than in control berries. The application of salicylate derivatives induced resistance to B. cinerea spoilage, since higher percentage of berries with no symptoms was observed and on the contrary, the highest percentages of berries were obtained in control grapes. All preharvest treatments with SA, ASA and MeSA alleviated postharvest disease caused by B. cinerea probably due to increasing levels of phenolic compounds and activity of antioxidant enzymes, although the best results were obtained with MeSA at 0.1 mM. Also, for this treatment and dose, higher quality properties, such as higher concentrations of ascorbic, succinic and fumaric acids, were observed compared with no treated-grapes.



中文翻译:

收获前施用水杨酸甲酯,乙酰水杨酸和水杨酸可通过刺激葡萄中的抗氧化系统减轻灰葡萄孢引起的疾病。

这项研究的主要目的是描述在两个食用葡萄品种中,水杨酸甲酯(MeSA),乙酰水杨酸(ASA)和水杨酸(SA)的收获前应用对灰葡萄孢引起的疾病减少的影响('Crimson'和“洋红色”)。根据先前的研究,MeSA和SA分别以0.1和0.01 mM应用于两个品种,而ASA在'Crimson'中以1 mM和'Magenta'在0.1 mM中应用。在收获时,确定了浆果的成熟质量属性,生物活性化合物和抗氧化酶。此外,葡萄还被人工接种了B. cinerea接种5天后,对孢子和浆果的视觉衰退发生率进行排名。与对照浆果相比,水杨酸酯收获前的处理导致处理后的总酸度,生物活性化合物含量和抗氧化酶活性更高。水杨酸酯衍生物的应用诱导了对灰质芽孢杆菌变质的抗性,因为观察到没有症状的浆果百分比更高,相反,在对照葡萄中获得了最高百分比的浆果。用SA,ASA和MeSA进行的所有收获前治疗均减轻了灰葡萄孢菌引起的收获后疾病尽管使用0.1 mM的MeSA可获得最佳结果,但这可能是由于酚类化合物水平的提高和抗氧化酶的活性所致。同样,对于这种处理和剂量,与未处理葡萄相比,观察到更高质量的特性,例如更高浓度的抗坏血酸,琥珀酸和富马酸。

更新日期:2020-08-21
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