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Aberrant FcγRIIb and FcγRIII expression on monocytes from patients with Behçet's disease.
Clinical Immunology ( IF 4.5 ) Pub Date : 2020-07-31 , DOI: 10.1016/j.clim.2020.108549
Linfang Huang 1 , Xin Yu 2 , Lu Li 2 , Jinjing Liu 2 , Xiuhua Wu 2 , Yan Zeng 3 , Xiangping Liao 4 , Wanli Liu 5 , Fengchun Zhang 2 , Xuan Zhang 2 , Hua Chen 2 , Wenjie Zheng 2
Affiliation  

Behçet's disease (BD) patients have abnormal FcγR polymorphisms, the implication of which remains elusive. We examined FcγRIIb expression on neutrophils, monocytes, B cells, natural killer cells, dendritic cells and T cells, and FcγRI and FcγRIII expression on monocytes in BD patients and healthy controls using flow cytometry. We further stimulated monocytes with IgG and (or) lipopolysaccharide (LPS) and measured IL-6 and TNF-α production by enzyme-linked immunosorbent assay. We found that BD monocytes expressed a lower level of FcγRIIb and a higher level of FcγRIII, which were correlated with erythrocyte sedimentation rate and C-reactive protein and were rescued after treatment. Furthermore, LPS- and IgG-stimulated BD monocytes produced higher levels of IL-6 and TNF-α than HC monocytes. In summary, we found that BD monocytes downregulated FcγRIIb expression and upregulated FcγRIII expression, which were correlated with disease activity and potentially contributed to monocyte hyperactivation in BD.



中文翻译:

白塞病患者单核细胞上的FcγRIIb和FcγRIII异常表达。

贝塞切氏病(BD)患者具有异常的FcγR多态性,其含义仍然难以捉摸。我们使用流式细胞仪检测了BD患者和健康对照组中性粒细胞,单核细胞,B细胞,自然杀伤细胞,树突细胞和T细胞上的FcγRIIb表达,以及单核细胞上的FcγRI和FcγRIII表达。我们进一步用IgG和(或)脂多糖(LPS)刺激了单核细胞,并通过酶联免疫吸附测定法测量了IL-6和TNF-α的产生。我们发现BD单核细胞表达较低水平的FcγRIIb和较高水​​平的FcγRIII,这与红细胞沉降率和C反应蛋白相关,并在治疗后得以挽救。此外,LPS和IgG刺激的BD单核细胞比HC单核细胞产生更高水平的IL-6和TNF-α。综上所述,

更新日期:2020-08-08
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