Bioresource Technology ( IF 11.4 ) Pub Date : 2020-07-31 , DOI: 10.1016/j.biortech.2020.123916 Bingli Liu 1 , Eladl E Eltanahy 2 , Hongwei Liu 3 , Elvis T Chua 4 , Skye R Thomas-Hall 4 , Taylor J Wass 4 , Kehou Pan 5 , Peer M Schenk 4
High-yielding microalgae present an important commodity to sustainably satisfy burgeoning food, feed and biofuel demands. Because algae-associated bacteria can significantly enhance or reduce yields, we isolated, identified and selected highly-effective “probiotic” bacterial strains associated with Nannochloropsis oceanica, a high-yielding microalga rich in eicosapentaenoic acid (EPA). Xenic algae growth was significantly enhanced by co-cultivation with ten isolated bacteria that improved culture density and biomass by 2.2- and 1.56-fold, respectively (1.39 × 108 cells mL−1; 0.82 g L−1). EPA contents increased up to 2.25-fold (to 39.68% of total fatty acids). Added probiotic bacteria possessed multiple growth-stimulating characteristics, including atmospheric nitrogen fixation, growth hormone production and phosphorous solubilization. Core N. oceanica-dominant bacterial microbiomes at different cultivation scales included Sphingobacteria, Flavobacteria (Bacteroidetes), and α, γ-Proteobacteria, and added probiotic bacteria could be maintained. We conclude that the supplementation with probiotic algae-associated bacteria can significantly enhance biomass and EPA production of N. oceanica.
中文翻译:
促进生长的细菌使微藻中的二十碳五烯酸产量提高了一倍。
高产微藻是可持续满足蓬勃发展的食品,饲料和生物燃料需求的重要商品。由于藻类相关细菌可以显着提高或降低产量,因此我们分离,鉴定和选择了与Nannochloropsis oceanica(一种富含二十碳五烯酸(EPA)的高产微藻)相关的高效“益生菌”细菌菌株。与十种分离的细菌共培养可显着提高Xenic藻类的生长,这些细菌分别将培养密度和生物量提高了2.2倍和1.56倍(1.39×10 8个 细胞mL -1; 0.82 g L -1)。EPA含量增加了2.25倍(达到总脂肪酸的39.68%)。添加的益生菌具有多种生长刺激特性,包括大气固氮,生长激素生成和磷增溶。芯N. OCEANICA -dominant细菌微生物组在不同栽培秤包括Sphingobacteria,黄杆菌(拟杆菌),和α,γ-变形菌,并添加益生菌可以维持。我们得出的结论是,益生菌藻类相关细菌的补充可以显着提高海洋猪笼草的生物量和EPA产量。