Abstract

The autocatalytic formation of a chromophore in a green fluorescent protein, which is actively used as a marker for visualizing processes in living systems, is an important example of multistage reactions in protein macromolecules. The kinetic, spectral, and X-ray structural experimental studies and supercomputer calculations by molecular modeling methods are devoted to the study of intermediates of the elementary reaction stages, which are grouped into cyclization and oxidation blocks. The article analyzes the results, compatible with the experimental data, of the structure of intermediates of the reactions of chromophore formation for the native protein form obtained through computer modeling, with the aim of including this information in the database of the reactions’ intermediates in enzymatic reactions.

中文翻译：

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绿色荧光蛋白中生色团形成的自催化反应的中间体

摘要

绿色荧光蛋白中发色团的自催化形成，积极地用作可视化生命系统过程的标记，是蛋白大分子中多阶段反应的重要例子。动力学，光谱和X射线结构实验研究以及通过分子建模方法进行的超级计算机计算致力于研究基本反应阶段的中间体，这些中间体分为环化和氧化嵌段。本文分析了通过计算机建模获得的天然蛋白质形式发色团形成反应中间体的结构与实验数据相符的结果，目的是将该信息纳入酶促反应中间体的数据库中反应。