Abstract—

Mitogenomic data can provide valuable information that can be used to elucidate and reveal the evolutionary history and phylogenetic relationships. Recently, mitogenomes generated using high-throughput sequencing technologies have become more applicable in association with advanced analytical tools. In this study, to provide mitogenomic data for the Indian crested porcupine and to disclose the intraspecific and interspecific relationships within the subgenus Hystrix, partial mitogenome (7.525 bp) of one sample from Turkey that included coding and noncoding regions was initially sequenced using the Illumina MiSeq platform. The structure of the obtained partial mitogenome was similar to those of the other mammals. The partial mitogenome sequence of the Turkish sample was used for phylogenetic analyses together with published mitochondrial sequences of the species within the subgenus Hystrix registered in GenBank. Two mitochondrial regions (CytB and D-loop available in GenBank) were analyzed using the Bayesian, Maximum Likelihood, and Neighbor-Joining methods. Phylogenetic analyses revealed three major phylogroups (Hystrix cristata, H. indica, and H. africaeaustralis) within the subgenus Hystrix. This partial mitogenome sequence was the first data for the Turkish porcupine belonging to the species H. indica. As genetic data for H. indica are already limited, the present study would contribute to further phylogenetic studies that would be performed using mitogenomic data of the Indian crested porcupine and other porcupines.

中文翻译：

---

土耳其印第安豪猪（Hystrix indica）的部分丝分裂基因组序列，以及Hystrix属的系统发育

摘要—

线粒体数据可以提供有价值的信息，可用于阐明和揭示进化史和系统发育关系。近来，使用高通量测序技术生成的有丝分裂基因组与先进的分析工具结合起来变得更加适用。在这项研究中，以提供印度凤头豪猪的线粒体数据，并揭示Hystrix亚属内的种内和种间关系首先，使用Illumina MiSeq平台对来自土耳其的一个样品的部分有丝分裂基因组（7.525 bp）进行了测序，该样品包括编码区和非编码区。获得的部分有丝分裂基因组的结构与其他哺乳动物的相似。将土耳其样品的部分有丝分裂基因组序列与在GenBank中注册的Hystrix亚属中物种的线粒体序列一起用于系统发育分析。使用贝叶斯方法，最大似然法和邻居加入法分析了两个线粒体区域（GenBank中提供的Cyt B和D环）。系统发育分析表明，三个主要的系统组（Hystrix cristata，H. indica和H. africaeaustralis）在Hystrix属中。这种部分线粒体基因序列是对属于物种土耳其豪猪第一数据H.籼稻。由于H稻的遗传数据已经有限，所以本研究将有助于进一步的系统发育研究，这些研究将使用印度凤头豪猪和其他豪猪的线粒体基因组数据进行。