MicroRNAs (miRs) have shown tremendous potential to act as therapeutic targets for cancer treatment. In this context, the present study was designed to investigate the potential of miR-143 in the treatment of breast cancer. Results showed that miR-143 to be significantly (P < 0.05) downregulated in breast cancer tissues and cell lines. The miR-143 has inhibitory effect on CAMA-1cell growth which was manifested as significant (P < 0.05) decline in loss of viability of cancer cells. The loss of cell viability was revealed to be due to the induction of apoptotic cell death as evident from acridine orange/ethidium bromide (AO/EB) and 4′,6-diamidino-2-phenylindole (DAPI) staining assays. The apoptotic cell percentage was found to be 35.7% in miR-143 mimics transfected in comparison to 6.4% in miR-NC transfected cells. The western blot analysis showed that miR-143 caused enhancement in Bax and suppression in Bcl-2 expression in CAMA-1 cells. The miR-143 also suppressed the bone metastasis of the CAMA-1 cells by suppressing the expression of Jag1 and deactivation of the Rho-signalling pathway. The transwell assays also showed considerable anti-metastatic effects of miR-143 on CAMA-1 cells. Taken together, miR-143 has growth inhibitory anti-metastatic effect on breast cancer and thus may prove beneficial in breast cancer treatment.

中文翻译：

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MicroRNA-143靶向MAPK3，以调节人乳腺癌细胞的增殖和骨转移。

MicroRNA（miRs）已显示出巨大的潜力，可以作为癌症治疗的靶标。在这种情况下，本研究旨在研究miR-143在乳腺癌治疗中的潜力。结果表明，miR-143在乳腺癌组织和细胞系中显着下调（P <0.05）。miR-143对CAMA-1细胞的生长具有抑制作用，表现为癌细胞活力丧失的显着（P <0.05）下降。从a啶橙/溴化乙锭（AO / EB）和4'，6-二mid基-2-苯基吲哚（DAPI）染色分析中可以明显看出，细胞活力的丧失是由于诱导凋亡细胞死亡。发现转染的miR-143模拟物中的凋亡细胞百分比为35.7％，而miR-NC转染的细胞中为6.4％。蛋白质印迹分析表明，miR-143导致CAMA-1细胞Bax增强，Bcl-2表达受到抑制。miR-143还通过抑制Jag1的表达和Rho信号通路的失活来抑制CAMA-1细胞的骨转移。Transwell分析还显示了miR-143对CAMA-1细胞的显着抗转移作用。两者合计，miR-143对乳腺癌具有抑制生长的抗转移作用，因此可能证明对乳腺癌的治疗有益。Transwell分析还显示了miR-143对CAMA-1细胞的显着抗转移作用。两者合计，miR-143对乳腺癌具有抑制生长的抗转移作用，因此可能证明对乳腺癌的治疗有益。Transwell分析还显示了miR-143对CAMA-1细胞的显着抗转移作用。两者合计，miR-143对乳腺癌具有抑制生长的抗转移作用，因此可能证明对乳腺癌的治疗有益。