The human CGGBP1 binds to GC-rich regions and interspersed repeats, maintains homeostasis of stochastic cytosine methylation and determines DNA-binding of CTCF. Interdependence between regulation of cytosine methylation and CTCF occupancy by CGGBP1 remains unknown. By analyzing methylated DNA-sequencing data obtained from CGGBP1-depleted cells, we report that some transcription factor-binding sites, including CTCF, resist stochastic changes in cytosine methylation. By analysing CTCF-binding sites we show that cytosine methylation changes at CTCF motifs caused by CGGBP1 depletion resist stochastic changes. These CTCF-binding sites are positioned at locations where the spread of cytosine methylation in cis depends on the levels of CGGBP1. Our findings suggest that CTCF occupancy and functions are determined by CGGBP1-regulated cytosine methylation patterns.

中文翻译：

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CGGBP1调节CTCF结合基序上的胞嘧啶甲基化可抵抗随机性。

人CGGBP1结合到富含GC的区域和散布的重复序列，维持随机胞嘧啶甲基化的稳态，并确定CTCF的DNA结合。CGGBP1调节胞嘧啶甲基化与CTCF占用之间的相互依赖性仍然未知。通过分析从CGGBP1耗尽的细胞获得的甲基化DNA测序数据，我们报告了一些转录因子结合位点，包括CTCF，可抵抗胞嘧啶甲基化的随机变化。通过分析CTCF结合位点，我们表明CGGBP1耗竭引起的CTCF基序处的胞嘧啶甲基化变化可抵抗随机变化。这些CTCF结合位点位于顺式胞嘧啶甲基化的扩散取决于CGGBP1的水平的位置。