The objectives of this study were to analyze and compare the proteomic and peptide profiles of mung bean (Vigna radiata) seeds and sprouts. Label-free proteomics and peptidomics technologies allowed the identification and relative quantification of proteins and peptides. There were 1918 and 1955 proteins identified in mung bean seeds and sprouts, respectively. The most common biological process of proteins in these two samples was the metabolic process, followed by cellular process and single-organism process. Their dominant molecular functions were catalytic activity, binding, and structural molecule activity, and the majority of them were the cell, cell part, and organelle proteins. These proteins were primarily involved in metabolic pathways, biosynthesis of secondary metabolites, and ribosome. PCA and HCA results indicated the proteomic profile varied significantly during mung bean germination. A total of 260 differential proteins between mung bean seeds and sprouts were selected based on their relative abundance, which were associated with the specific metabolism during seed germination. There were 2364 peptides identified and 76 potential bioactive peptides screened based on the in silico analysis. Both the types and concentration of the peptides in mung bean sprouts were higher than those in seeds, and the content of bioactive peptides in mung bean sprouts was deduced to be higher.

中文翻译：

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绿豆种子和芽的蛋白质组和肽谱的鉴定和比较。


本研究的目的是分析和比较绿豆（Vigna radiata）种子和芽的蛋白质组和肽谱。无标记蛋白质组学和肽组学技术可以对蛋白质和肽进行鉴定和相对定量。在绿豆种子和豆芽中分别鉴定出 1918 和 1955 个蛋白质。这两个样品中蛋白质最常见的生物过程是代谢过程，其次是细胞过程和单一生物体过程。它们的主要分子功能是催化活性、结合和结构分子活性，其中大多数是细胞、细胞部分和细胞器蛋白。这些蛋白质主要参与代谢途径、次生代谢物的生物合成和核糖体。 PCA和HCA结果表明绿豆萌发过程中蛋白质组谱变化显着。根据绿豆种子和芽苗的相对丰度，筛选出260个差异蛋白，这些差异蛋白与种子萌发过程中的特定代谢相关。根据计算机分析，鉴定出 2364 种肽，筛选出 76 种潜在生物活性肽。绿豆芽中肽的种类和浓度均高于种子中的肽，推测绿豆芽中生物活性肽的含量较高。