The current study was undertaken to isolate and identify anti-inflammatory and antiallergic active compounds in the ethyl acetate and butanol fractions of an ethanolic extract of the aerial parts of Stellera chamaejasme (SCAE). A new maltol glucoside, maltol 3-(6″-(2-(E)-butenoyl)-glucoside) and benzoic acid, daphnoretin (a coumarin), 6 flavonoids (apigenin, genkwanin, taxifolin, apigenin 7-O-glucoside, luteolin 7-O-sambubioside, genkwanin 5-O-glucoside), and 2 lignans (matairesinoside and lariciresinol) were isolated from SCAE by chromatographic separation. Their chemical structures were elucidated by analyzing spectroscopic data, including 1-dimensional and 2-dimensional nuclear magnetic resonance (Fig1). Maltol 3-(6″-(2-(E)-butenoyl)-glucoside) significantly inhibited β-hexosaminidase release by 85.5% in immunoglobulin E and dinitrophenyl/bovine serum albumin (DNP/BSA)-induced RBL-2H3 cells. Benzoic acid from S. chamaejasme inhibited β-hexosaminidase release (by 80.2%) in IgE and DNP/BSA-induced RBL-2H3 cells and interleukin-4 messenger ribonucleic acid expression (by 21.9% inhibition) in propidium iodide-induced RBL-2H3 cells.

目前的研究是为了分离和鉴定Stellera chamaejasme（SCAE）地上部分乙醇提取物中的乙酸乙酯和丁醇级分中的抗炎和抗过敏活性化合物。一种新的麦芽酚葡糖苷，麦芽酚3-（6″-（2-（E）-丁烯酰基）-葡糖苷）和苯甲酸，瑞香素（一种香豆素），6种类黄酮（芹菜素，genkwanin，滑石粉，芹菜素7- O-葡糖苷，木犀草素7- O-桑布比苷，Genkwanin 5- O-葡糖苷）和2种木脂素（苦参皂苷和lariciresinol）通过色谱分离从SCAE中分离出来。通过分析光谱数据，包括一维和二维核磁共振，阐明了它们的化学结构（图1）。麦芽酚3-（6″-（2-（E）-丁烯酰）-葡萄糖苷）在免疫球蛋白E和二硝基苯基/牛血清白蛋白（DNP / BSA）诱导的RBL-2H3细胞中显着抑制β-己糖胺酶释放达85.5％。从苯甲酸狼毒在碘化丙啶诱导的RBL-2H3中IgE和DNP /抑制β氨基己糖苷酶释放（由80.2％）BSA诱发的RBL-2H3细胞和白细胞介素-4信使核糖核酸的表达（21.9％抑制）细胞。