The R2R3-MYB genes comprise one of the largest transcription factor gene families in plants, playing regulatory roles in plant-specific developmental processes, defense responses and metabolite accumulation. To date MYB family genes have not yet been comprehensively identified in the major staple fruit crop banana. In this study, we present a comprehensive, genome-wide analysis of the MYB genes from Musa acuminata DH-Pahang (A genome). A total of 285 R2R3-MYB genes as well as genes encoding three other classes of MYB proteins containing multiple MYB repeats were identified and characterised with respect to structure and chromosomal organisation. Organ- and development-specific expression patterns were determined from RNA-seq data. For 280 M. acuminata MYB genes for which expression was found in at least one of the analysed samples, a variety of expression patterns were detected. The M. acuminata R2R3-MYB genes were functionally categorised, leading to the identification of seven clades containing only M. acuminata R2R3 MYBs. The encoded proteins may have specialised functions that were acquired or expanded in Musa during genome evolution. This functional classification and expression analysis of the MYB gene family in banana establishes a solid foundation for future comprehensive functional analysis of MaMYBs and can be utilized in banana improvement programmes.

中文翻译：

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香蕉（Musa acuminata）中的R2R3-MYB基因家族：全基因组的鉴定，分类和表达模式

R2R3-MYB基因构成植物中最大的转录因子基因家族之一，在植物特异性发育过程，防御反应和代谢产物积累中发挥调节作用。迄今为止，尚未在主要的主食水果作物香蕉中全面鉴定出MYB家族基因。在这项研究中，我们提出了从Musa acuminata DH-Pahang（A基因组）MYB基因的全面，全基因组的分析。鉴定并鉴定了总共285个R2R3-MYB基因以及编码含有多个MYB重复序列的其他三类MYB蛋白的基因，并就结构和染色体组织进行了表征。从RNA-seq数据确定器官和发育特异性的表达模式。对于在至少一个分析样本中发现表达的280个尖锐梭菌MYB基因，检测到多种表达模式。按功能分类M. acuminata R2R3-MYB基因，从而鉴定出仅包含M. acuminata R2R3 MYBs的七个进化枝。编码的蛋白质可能具有在基因组进化过程中在Musa中获得或扩展的专门功能。香蕉中MYB基因家族的功能分类和表达分析为MaMYB的未来综合功能分析奠定了坚实的基础，可用于香蕉改良计划。