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Single-shot Ad26 vaccine protects against SARS-CoV-2 in rhesus macaques
Nature ( IF 64.8 ) Pub Date : 2020-07-30 , DOI: 10.1038/s41586-020-2607-z Noe B Mercado 1 , Roland Zahn 2 , Frank Wegmann 2 , Carolin Loos 3, 4 , Abishek Chandrashekar 1 , Jingyou Yu 1 , Jinyan Liu 1 , Lauren Peter 1 , Katherine McMahan 1 , Lisa H Tostanoski 1 , Xuan He 1 , David R Martinez 5 , Lucy Rutten 2 , Rinke Bos 2 , Danielle van Manen 2 , Jort Vellinga 2 , Jerome Custers 2 , Johannes P Langedijk 2 , Ted Kwaks 2 , Mark J G Bakkers 2 , David Zuijdgeest 2 , Sietske K Rosendahl Huber 2 , Caroline Atyeo 3, 6 , Stephanie Fischinger 3, 6 , John S Burke 3 , Jared Feldman 3, 6 , Blake M Hauser 3, 6 , Timothy M Caradonna 3, 6 , Esther A Bondzie 1 , Gabriel Dagotto 1, 6 , Makda S Gebre 1, 6 , Emily Hoffman 1 , Catherine Jacob-Dolan 1, 6 , Marinela Kirilova 1 , Zhenfeng Li 1 , Zijin Lin 1 , Shant H Mahrokhian 1 , Lori F Maxfield 1 , Felix Nampanya 1 , Ramya Nityanandam 1 , Joseph P Nkolola 1 , Shivani Patel 1 , John D Ventura 1 , Kaylee Verrington 1 , Huahua Wan 1 , Laurent Pessaint 7 , Alex Van Ry 7 , Kelvin Blade 7 , Amanda Strasbaugh 7 , Mehtap Cabus 7 , Renita Brown 7 , Anthony Cook 7 , Serge Zouantchangadou 7 , Elyse Teow 7 , Hanne Andersen 7 , Mark G Lewis 7 , Yongfei Cai 8 , Bing Chen 8, 9 , Aaron G Schmidt 3, 6, 9 , R Keith Reeves 1 , Ralph S Baric 5 , Douglas A Lauffenburger 4 , Galit Alter 3, 9 , Paul Stoffels 2 , Mathai Mammen 2 , Johan Van Hoof 2 , Hanneke Schuitemaker 2 , Dan H Barouch 1, 3, 6, 9
Nature ( IF 64.8 ) Pub Date : 2020-07-30 , DOI: 10.1038/s41586-020-2607-z Noe B Mercado 1 , Roland Zahn 2 , Frank Wegmann 2 , Carolin Loos 3, 4 , Abishek Chandrashekar 1 , Jingyou Yu 1 , Jinyan Liu 1 , Lauren Peter 1 , Katherine McMahan 1 , Lisa H Tostanoski 1 , Xuan He 1 , David R Martinez 5 , Lucy Rutten 2 , Rinke Bos 2 , Danielle van Manen 2 , Jort Vellinga 2 , Jerome Custers 2 , Johannes P Langedijk 2 , Ted Kwaks 2 , Mark J G Bakkers 2 , David Zuijdgeest 2 , Sietske K Rosendahl Huber 2 , Caroline Atyeo 3, 6 , Stephanie Fischinger 3, 6 , John S Burke 3 , Jared Feldman 3, 6 , Blake M Hauser 3, 6 , Timothy M Caradonna 3, 6 , Esther A Bondzie 1 , Gabriel Dagotto 1, 6 , Makda S Gebre 1, 6 , Emily Hoffman 1 , Catherine Jacob-Dolan 1, 6 , Marinela Kirilova 1 , Zhenfeng Li 1 , Zijin Lin 1 , Shant H Mahrokhian 1 , Lori F Maxfield 1 , Felix Nampanya 1 , Ramya Nityanandam 1 , Joseph P Nkolola 1 , Shivani Patel 1 , John D Ventura 1 , Kaylee Verrington 1 , Huahua Wan 1 , Laurent Pessaint 7 , Alex Van Ry 7 , Kelvin Blade 7 , Amanda Strasbaugh 7 , Mehtap Cabus 7 , Renita Brown 7 , Anthony Cook 7 , Serge Zouantchangadou 7 , Elyse Teow 7 , Hanne Andersen 7 , Mark G Lewis 7 , Yongfei Cai 8 , Bing Chen 8, 9 , Aaron G Schmidt 3, 6, 9 , R Keith Reeves 1 , Ralph S Baric 5 , Douglas A Lauffenburger 4 , Galit Alter 3, 9 , Paul Stoffels 2 , Mathai Mammen 2 , Johan Van Hoof 2 , Hanneke Schuitemaker 2 , Dan H Barouch 1, 3, 6, 9
Affiliation
A safe and effective vaccine for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) may be required to end the coronavirus disease 2019 (COVID-19) pandemic1-8. For global deployment and pandemic control, a vaccine that requires only a single immunization would be optimal. Here we show the immunogenicity and protective efficacy of a single dose of adenovirus serotype 26 (Ad26) vector-based vaccines expressing the SARS-CoV-2 spike (S) protein in nonhuman primates. Fifty-two rhesus macaques were immunized with Ad26 vectors encoding S variants or sham control and were challenged with SARS-CoV-2 by the intranasal and intratracheal routes9,10. The optimal Ad26 vaccine induced robust neutralizing antibody responses and provided complete or near-complete protection in bronchoalveolar lavage and nasal swabs following SARS-CoV-2 challenge. Vaccine-elicited neutralizing antibody titres correlated with protective efficacy, suggesting an immune correlate of protection. These data demonstrate robust single-shot vaccine protection against SARS-CoV-2 in nonhuman primates. The optimal Ad26 vector-based vaccine for SARS-CoV-2, termed Ad26.COV2.S, is currently being evaluated in clinical trials.
中文翻译：
单次注射 Ad26 疫苗可保护恒河猴免受 SARS-CoV-2 侵害
可能需要一种安全有效的严重急性呼吸系统综合症冠状病毒 2 (SARS-CoV-2) 疫苗来结束 2019 年冠状病毒病 (COVID-19) 大流行 1-8。对于全球部署和大流行控制，只需要单次免疫的疫苗将是最佳选择。在这里，我们展示了单剂量基于腺病毒血清型 26 (Ad26) 载体的疫苗在非人灵长类动物中表达 SARS-CoV-2 刺突 (S) 蛋白的免疫原性和保护功效。用编码 S 变体或假对照的 Ad26 载体对 52 只恒河猴进行免疫，并通过鼻内和气管内途径用 SARS-CoV-2 进行攻击9,10。最佳的 Ad26 疫苗诱导了强烈的中和抗体反应，并在 SARS-CoV-2 攻击后在支气管肺泡灌洗液和鼻拭子中提供了完全或接近完全的保护。疫苗引发的中和抗体滴度与保护功效相关，表明保护与免疫相关。这些数据证明了非人灵长类动物对 SARS-CoV-2 的单次注射疫苗具有强大的保护作用。SARS-CoV-2 的最佳 Ad26 载体疫苗称为 Ad26.COV2.S，目前正在临床试验中进行评估。
更新日期：2020-07-30
中文翻译：
单次注射 Ad26 疫苗可保护恒河猴免受 SARS-CoV-2 侵害
可能需要一种安全有效的严重急性呼吸系统综合症冠状病毒 2 (SARS-CoV-2) 疫苗来结束 2019 年冠状病毒病 (COVID-19) 大流行 1-8。对于全球部署和大流行控制，只需要单次免疫的疫苗将是最佳选择。在这里，我们展示了单剂量基于腺病毒血清型 26 (Ad26) 载体的疫苗在非人灵长类动物中表达 SARS-CoV-2 刺突 (S) 蛋白的免疫原性和保护功效。用编码 S 变体或假对照的 Ad26 载体对 52 只恒河猴进行免疫，并通过鼻内和气管内途径用 SARS-CoV-2 进行攻击9,10。最佳的 Ad26 疫苗诱导了强烈的中和抗体反应，并在 SARS-CoV-2 攻击后在支气管肺泡灌洗液和鼻拭子中提供了完全或接近完全的保护。疫苗引发的中和抗体滴度与保护功效相关，表明保护与免疫相关。这些数据证明了非人灵长类动物对 SARS-CoV-2 的单次注射疫苗具有强大的保护作用。SARS-CoV-2 的最佳 Ad26 载体疫苗称为 Ad26.COV2.S，目前正在临床试验中进行评估。
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