Instant Self-Assembly Peptide Hydrogel Encapsulation with Fibrous Alginate by Microfluidics for Infected Wound Healing,ACS Biomaterials Science & Engineering

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Instant Self-Assembly Peptide Hydrogel Encapsulation with Fibrous Alginate by Microfluidics for Infected Wound Healing
ACS Biomaterials Science & Engineering ( IF 5.4 ) Pub Date : 2020-07-30 , DOI: 10.1021/acsbiomaterials.0c00581
Tianyu Cui ₁ , Xiuping Li ₁ , Suyun He ₁ , Danhan Xu ₁ , Li Yin ₁ , Xiaoling Huang ₁ , Siwei Deng ₁ , Wanqing Yue _{1,

2} , Wenying Zhong ₁

Affiliation

Infected wounds caused by persistent inflammation exhibit poor vascularization and cellular infiltration. In order to rapidly control the inflammatory effect and accelerate wound healing, it is necessary to develop a novel drug vehicle addressing the need for infected wounds. Herein, we developed a novel dual-drug delivery system with micrometer-scale alginate fibers encapsulated in instant self-assembly peptide hydrogel. Short peptides with the sequence of Nap-Gly-Phe-Phe-Lys-His (Nap-GFFKH) could self-assemble outside the microfluidic-based alginate microfibers in weak acidic solution (pH ≈ 6.0) within 5 s. The gelation condition is close to the pH environment of the human skin. We further constructed recombinant bovine basic fibroblast growth factor (FGF-2) in fibrous alginate, which was encapsulated in antibiotic-loaded peptide hydrogel. The dual-drug delivery system exhibited good mechanical property and sustained release profiles, where antibiotic could be rapidly released from the peptide hydrogel, while the growth factor could be gradually released within 7 days. Both in vitro antibacterial experiments and in vivo animal experiments confirmed that such a dual-drug delivery system has good antibacterial activity and enhances wound healing property. We suggested that the dual-drug delivery system could be potentially applied for controlled drug release in infected wound healing, drug combination for melanoma therapy, and tissue engineering.

中文翻译：

藻酸纤维微流控技术即时包裹自组装肽水凝胶，用于伤口愈合

由持续性炎症引起的感染伤口表现出不良的血管形成和细胞浸润。为了快速控制炎症作用并加速伤口愈合，有必要开发一种新型的药物载体来解决感染伤口的需要。在这里，我们开发了一种新型的双重药物输送系统，该系统将微米级藻酸盐纤维封装在即时自组装肽水凝胶中。具有Nap-Gly-Phe-Phe-Lys-His序列的短肽（Nap-GFFKH）可以在5 s内在弱酸性溶液（pH≈6.0）中的基于微流体的藻酸盐微纤维外自组装。胶凝条件接近于人类皮肤的pH环境。我们进一步在纤维藻酸盐中构建了重组牛碱性成纤维细胞生长因子（FGF-2），将其封装在载有抗生素的肽水凝胶中。双药递送系统表现出良好的机械性能和持续释放曲线，其中抗生素可以从肽水凝胶中快速释放，而生长因子可以在7天内逐渐释放。都体外抗菌实验和体内动物实验证实了这种双重药物递送系统具有良好的抗菌活性并增强了伤口的愈合性能。我们建议双重药物输送系统可潜在地用于受感染伤口愈合，黑色素瘤治疗的药物组合和组织工程学中的受控药物释放。

更新日期：2020-09-14

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