Host–Guest-Mediated Epitope Presentation on Self-Assembled Peptide Amphiphile Hydrogels,ACS Biomaterials Science & Engineering

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Host–Guest-Mediated Epitope Presentation on Self-Assembled Peptide Amphiphile Hydrogels
ACS Biomaterials Science & Engineering ( IF 5.4 ) Pub Date : 2020-07-29 , DOI: 10.1021/acsbiomaterials.0c00549
Carlos Redondo-Gómez _{1,

2} , Soraya Padilla-Lopategui _{1,

2} , Helena S. Azevedo _{1,

2} , Alvaro Mata _{1,

2,

3,

4,

5}

Affiliation

A key feature in biomaterial design is the incorporation of bioactive signals into artificial constructs to stimulate tissue regeneration. Most currently used hydrogel cell culture systems depend on the covalent attachment of extracellular matrix (ECM)-derived peptides to either macromolecular units or smaller self-assembling building blocks, thereby restricting biosignal presentation and adaptability. However, new ways to rationally incorporate adhesion epitopes through noncovalent interactions would offer opportunities to better recreate the dynamic and reversible nature of the native ECM. Here, we report on a noncovalent epitope presentation approach mediated by host–guest interactions. Using peptide amphiphile hydrogels, we demonstrate that the adamantane/β-cyclodextrin pair can be used to anchor RGDS cell adhesion signals onto self-assembled hydrogels via host–guest interactions. We evaluate hydrogel morphological and rheological properties as well as fibroblast attachment, organization, and spreading when cultured atop these scaffolds. This host–guest-mediated epitope display might lead to new self-assembling hydrogels for improved cell culture applications in fields such as tissue engineering and regenerative medicine.

中文翻译：

自组装肽两亲水凝胶的宿主-来宾介导的抗原决定簇表达

生物材料设计的关键特征是将生物活性信号掺入人工构建物中以刺激组织再生。当前最常用的水凝胶细胞培养系统取决于细胞外基质（ECM）衍生肽与大分子单元或较小的自组装构件的共价结合，从而限制了生物信号的表达和适应性。但是，通过非共价相互作用合理掺入粘附表位的新方法将提供机会，以更好地重现天然ECM的动态和可逆性质。在这里，我们报道了一种由宿主-客体相互作用介导的非共价抗原决定簇呈递方法。使用肽两亲水凝胶，通过主人与客人的互动。当在这些支架上培养时，我们评估水凝胶的形态和流变特性以及成纤维细胞的附着，组织和扩散。这种由宿主-客体介导的表位展示可能会导致新的自组装水凝胶，以改善组织工程和再生医学等领域的细胞培养应用。

更新日期：2020-09-14

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