Abstract

Drug-loaded nanoparticles have been widely used in the field of tumor treatment due to their low side effects and reduced frequency of administration. In this study, keratin-dopamine conjugate was first synthesized by amidation reaction and then formed nanoparticles by self-polymerization of dopamine segment. Keratin-dopamine conjugate nanoparticles (KNPs) exhibited pH and glutathione (GSH) dual responsiveness in the simulated tumor environment. These nanoparticles were able to load anti-cancer drug doxorubicin (DOX) through electrostatic interactions and hydrogen bonds. These drug-loaded KNPs (DKNPs) exhibited controlled drug release in a tumor simulation environment. Meanwhile, DKNPs performed a stronger inhibitory effect on tumor cells compared with human normal tissue cells. Based on the above results, keratin-dopamine conjugate based drug carriers had a broad prospect in the field of cancer treatment.

摘要

载药纳米颗粒由于其低副作用和降低给药频率而被广泛应用于肿瘤治疗领域。在这项研究中，角蛋白-多巴胺缀合物首先通过酰胺化反应合成，然后通过多巴胺片段的自聚合形成纳米颗粒。角蛋白-多巴胺共轭纳米粒子 (KNPs) 在模拟肿瘤环境中表现出 pH 值和谷胱甘肽 (GSH) 双重反应性。这些纳米颗粒能够通过静电相互作用和氢键负载抗癌药物阿霉素（DOX）。这些载药 KNPs (DKNPs) 在肿瘤模拟环境中表现出受控的药物释放。同时，与人体正常组织细胞相比，DKNPs对肿瘤细胞的抑制作用更强。根据以上结果，