ABSTRACT The use of plant cell suspension culture systems has demonstrated to be highly suitable for metabolomics investigations of inducible defense responses. Here we report on sorghum cell suspension cultures that were elicited with purified lipopolysaccharides from the sorghum pathogen Burkholderia andropogonis, to activate metabolic pathways involved in the chemical defenses of the plant. Metabolomic analysis using liquid chromatography coupled to mass spectrometry identified a resorcinol phenolic lipid, annotated as sorgoleone, as one of the biomarkers associated with the LPS-induced response. Sorgoleone is a semiochemical and an allelochemical, synthesized by specialized root hair cells and the major component of the hydrophobic root exudate of sorghum. Its detection in undifferentiated cells might indicate a previously undescribed role for this phytochemical in plant defense responses.

中文翻译：

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脂多糖触发双色高粱细胞培养物中化感化学物质山梨醇的合成

摘要 植物细胞悬浮培养系统的使用已证明非常适合诱导防御反应的代谢组学研究。在这里，我们报告了用来自高粱病原体伯克霍尔德菌 (Burkholderia andropogonis) 的纯化脂多糖引发的高粱细胞悬浮培养物，以激活参与植物化学防御的代谢途径。使用液相色谱与质谱联用进行的代谢组学分析确定了一种间苯二酚酚类脂类，标注为 sorgoleone，是与 LPS 诱导反应相关的生物标志物之一。Sorgoleone 是一种化学信息素和化感物质，由特化的根毛细胞合成，是高粱疏水性根系分泌物的主要成分。