ABSTRACT

Currently, testing for coronavirus is performed with time and personnel consuming PCR assays. The aim of this study was to evaluate the sensitivity, specificity and capacity of a fully automated, random access high-throughput real-time PCR-based diagnostic platform for the detection of SARS-CoV-2. The NeuMoDx N96 system displayed an equal or better detection rate for SARS-CoV-2 compared with the LightCycler 480II system and showed a specificity of 100%. The median PCR run time for all 28 PCR runs was 91 (IQR 84–97) minutes. The capacity of the NeuMoDx N96 could easily surpass the capacity of most currently used molecular test systems and significantly reduce the turn-around time.

摘要

当前，冠状病毒的测试需要花费大量时间和人员进行PCR分析。这项研究的目的是评估用于检测SARS-CoV-2的全自动，随机访问，高通量，基于实时PCR的诊断平台的敏感性，特异性和能力。所述NeuMoDx N96系统显示与相比，SARS-CoV的-2同等或更好的检测率的LightCycler 480II系统和显示出100％的特异性。所有28个PCR运行的中值PCR运行时间为91（IQR 84–97）分钟。在容量NeuMoDx N96可以轻松超越大多数目前使用的分子检测系统的容量和显著减少周转时间。