Abstract

Lavandula angustifolia Mill. (lavender) and Origanum heracleoticum L. (greek oregano) are aromatic plants used in traditional medicine for relief of convulsion, anxiety, insomnia and in the treatment of neurological disorders. On the basis of literature, we evaluated the changing in glutathione enzymatic activities provoked by the essential oils and pure components, linalool, carvacrol and limonene to study the mechanisms of action, responsible for several activities. Activities of SOD (superoxide dismutase) and GPx (glutathione peroxidase) tend to increase respect to control. SOD maximal increase reached up to 117% for carvacrol and limonene. Increase in GPx activity reached even up to value 1.229 for origanum oil in comparison to control 0.125 μkat/mg prot. Activities of GR (glutathione reductase), except for lavender, showed a biphasic response. Like for GR, the administration of compounds, resulted in a biphasic response in GST (glutathione-S-transferase) activities (with a consistent increase in activity at concentration 125 μg/mL for all compounds except lavender). Moreover, the changes in GSH (reduced glutathione), are no significant for different concentrations of essential oils. So, the biological properties of essential oils and specifically, the antioxidant ones, can be related to their capacity to modify the glutathione enzymatic activities.

摘要

薰衣草磨坊。（薰衣草）和牛至L.（希腊牛至）是一种芳香植物，在传统医学中用于缓解惊厥、焦虑、失眠和治疗神经系统疾病。在文献的基础上，我们评估了由精油和纯成分芳樟醇、香芹酚和柠檬烯引起的谷胱甘肽酶活性的变化，以研究其作用机制，负责多种活动。SOD（超氧化物歧化酶）和 GPx（谷胱甘肽过氧化物酶）的活性倾向于增加对对照的尊重。香芹酚和柠檬烯的 SOD 最大增加达到 117%。与对照 0.125 μkat/mg prot 相比，牛至油的 GPx 活性增加甚至高达 1.229。除薰衣草外，GR（谷胱甘肽还原酶）的活性表现出双相反应。与 GR 一样，化合物的给药，导致 GST（谷胱甘肽-S-转移酶）活性的双相反应（除薰衣草外的所有化合物，在浓度为 125 μg/mL 时活性持续增加）。此外，对于不同浓度的精油，GSH（还原型谷胱甘肽）的变化并不显着。因此，精油的生物学特性，特别是抗氧化剂，可能与它们改变谷胱甘肽酶活性的能力有关。