Abstract

Stem barks of Boswellia dalzielii are used traditionally for the treatment of various bacterial infections. A bioassay guided fractionation of the MeOH-CH₂Cl₂ (1/1, v/v) stem barks extract led to the isolation of fourteen compounds 1–14, identified based on spectroscopic data. Dalzienoside (1) is reported here for the first time. The broth microdilution method was used to evaluate the antibacterial activity of the crude extract, fractions and compounds against six bacterial strains. The crude extract exhibited moderate antibacterial activity with MIC of 250 μL/ml; two fractions showed significant activities with MICs ranging from 7.8 to 125 μg/ml, while α-boswellic acid (2), β-boswellic acid (3), acetyl-11-keto-β-boswellic acid (4) from these fractions exhibited strong activities with MIC value of 3.125 µg/mL against Staphylococcus aureus, Salmonella typhi, Enterobacter cloacae, Streptococcus pneumonia and Pseudomonia aeruginosa. This study gives insight into the antibacterial constituents of the stem bark of B. dalzielii and justifies its use in ethnomedicine.

摘要

Boswellia dalzielii 的茎皮传统上用于治疗各种细菌感染。对 MeOH-CH ₂ Cl ₂ (1/1, v/v) 茎皮提取物进行生物测定引导分馏后，分离出了 14 种化合物1-14，根据光谱数据进行鉴定。Dalzienoside ( 1)是首次在此报道。肉汤微量稀释法用于评估粗提物、馏分和化合物对六种细菌菌株的抗菌活性。粗提物表现出中等抑菌活性，MIC为250 μL/ml；两个部分显示出显着的活性，MIC 范围为 7.8 至 125 μg/ml，而α-乳香酸（2 )、β-乳香酸 ( 3 )、乙酰-11-酮-β-乳香酸 ( 4 ) 表现出很强的活性，对金黄色葡萄球菌、伤寒沙门氏菌、阴沟肠杆菌、肺炎链球菌的MIC 值为 3.125 µg/mL和铜绿假单胞菌。本研究深入了解B. dalzielii茎皮的抗菌成分，并证明其在民族医学中的应用是合理的。