Peripheral nerve injury is a common clinical neurological disease. In our previous study, highly oriented poly (L-lactic acid) (PLLA)/soy protein isolate (SPI) nanofiber nerve conduits were constructed and exhibited a certain repair capacity for peripheral nerve injury. In order to further improve their nerve repairing efficiency, the bone mesenchymal stem cells (BMSCs) overexpressing brain derived neurotrophic factor (BDNF) and glial cell line-derived neurotrophic factor (GDNF) were introduced into the conduits as seed cells and then were used to repair the 10-mm sciatic nerve defects in rats. The nerve repair efficiency of the functional nerve conduits was evaluated by gait experiment, electrophysiological test, and a series of assays such as hemotoxylin-eosin (HE) staining, immunofluorescence staining, toluidine blue (TB) staining, transmission electron microscopy (TEM) observation of regenerated nerve and Masson’s trichrome staining of gastrocnemius muscle. The results showed that the conduits containing BMSCs overexpressing BDNF and GDNF double-factors group had better nerve repairing efficiency than blank BMSCs and single BDNF or GDNF factor groups, and superior to autografts group in some aspects. These data demonstrated that BDNF and GDNF produced by BMSCs could synergistically promote peripheral nerve repair. This study shed a new light on the conduits and stem cells-based peripheral nerve repair.

中文翻译：

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脑源性神经营养因子和胶质细胞源性神经营养因子转染的骨间充质干细胞用于修复周围神经损伤


周围神经损伤是临床常见的神经系统疾病。我们前期的研究构建了高度定向的聚左旋乳酸（PLLA）/大豆分离蛋白（SPI）纳米纤维神经导管，并对周围神经损伤表现出一定的修复能力。为了进一步提高其神经修复效率，将过表达脑源性神经营养因子（BDNF）和胶质细胞源性神经营养因子（GDNF）的骨间充质干细胞（BMSC）作为种子细胞引入导管中，然后用于修复大鼠 10 毫米坐骨神经缺损。通过步态实验、电生理测试以及苏木精-伊红（HE）染色、免疫荧光染色、甲苯胺蓝（TB）染色、透射电镜（TEM）观察等一系列测定来评估功能神经导管的神经修复效率再生神经的观察和腓肠肌的马森三色染色。结果显示，含有过表达BDNF和GDNF双因子组的BMSC的神经修复效率优于空白BMSC和单BDNF或GDNF因子组，并且在某些方面优于自体移植组。这些数据表明BMSCs产生的BDNF和GDNF可以协同促进周围神经修复。这项研究为导管和基于干细胞的周围神经修复提供了新的线索。