We investigated the expression pattern of four major starch genes at different seed developmental stages in the radiation-bred amaranth variety “Pribina” (Amaranthus cruentus L.) and corresponding control genotype “Ficha” (Amaranthus cruentus L.). Two platforms were used and compared for the gene expression analysis of GBSSI, SSSI, SBE, and DBE amaranth genes, including a standard quantitative real-time PCR (qPCR) technique and relatively novel droplet digital PCR (ddPCR) assay. In our conditions, both methods showed great accuracy and revealed higher expression of the investigated genes in the mutant variety than in the control genotype. Here we report for the first time, a ddPCR gene expression assay for the cultivated grain amaranth, as the most important group of the species in the genus Amaranthus.

中文翻译：

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与实时荧光定量PCR相比，辐射发达的Var菜品种中与淀粉相关的基本基因的数字绝对基因表达分析。

我们调查了辐射育a菜品种“ Pribina”（Amaranthus cruentus L.）和相应的对照基因型“ Ficha”（Amaranthus cruentus L.）在种子发育不同阶段四个主要淀粉基因的表达模式。使用了两个平台并比较了GBSSI，SSSI，SBE和DBE的基因表达分析。mar菜基因，包括标准的定量实时PCR（qPCR）技术和相对新颖的液滴数字PCR（ddPCR）分析。在我们的条件下，这两种方法均显示出很高的准确性，并且揭示了所研究基因在突变体品种中的表达高于对照基因型。在这里，我们首次报道了作为grain菜属最重要物种的栽培grain菜的ddPCR基因表达测定。