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The Determination of Antimicrobial Susceptibility by MIC and Epidemiological Cut‐Off Values and the Detection of Resistance Genes in Aeromonas Species Isolated from Cultured Fish
Letters in Applied Microbiology ( IF 2.0 ) Pub Date : 2020-09-01 , DOI: 10.1111/lam.13363
M Duman 1 , I B Saticioglu 2 , S Altun 1
Affiliation  

The present study was aimed at determining antimicrobial susceptibility by a CLSI standard microdilution testing protocol and detecting the resistance genes of motile Aeromonas species isolated from cultured fish. The importance of the minimum inhibitory concentrations was assessed based on statistically determined epidemiological cut‐off values calculated by normalized resistance analysis. Unfortunately, CLSI epidemiological cut‐off values are available only for Aeromonas salmonicida, and there is no further detailed data on Aeromonas isolated from aquatic animals. The antimicrobial susceptibilities of pre‐identified motile Aeromonas species to florfenicol, tetracycline and sulfamethoxazole were determined by calculating epidemiological cut‐off values with fully automated and freely available Excel spreadsheets, applying the normalized resistance interpretation (NRI) method. Furthermore, the presence of the antimicrobial resistance genes floR, tetA, tetB, tetC, tetD, tetE, tetH, sulI, sulII and sulIII was detected by PCR analysis and confirmed by sequence analysis. The presence of up to six different genes (multiple antimicrobial resistance) was determined in the Aeromonas isolates.

中文翻译:

通过 MIC 和流行病学临界值确定抗菌药物敏感性,并检测从养殖鱼中分离的气单胞菌物种中的抗性基因

本研究旨在通过 CLSI 标准微量稀释测试方案确定抗菌药物敏感性,并检测从养殖鱼中分离的活动气单胞菌物种的抗性基因。最低抑菌浓度的重要性是根据统计确定的流行病学临界值通过归一化耐药分析计算得出的。不幸的是,CLSI 流行病学临界值仅适用于杀鲑气单胞菌,并且没有关于从水生动物中分离的气单胞菌的进一步详细数据。预先确定的活动气单胞菌对氟苯尼考、四环素和磺胺甲恶唑的抗菌敏感性是通过使用全自动和免费提供的 Excel 电子表格计算流行病学临界值来确定的,应用归一化电阻解释 (NRI) 方法。此外,通过PCR分析检测并通过序列分析确认了抗菌素抗性基因floR、tetA、tetB、tetC、tetD、tetE、tetH、sulI、sulII和sulIII的存在。在气单胞菌分离物中测定了多达六个不同基因(多重抗菌素耐药性)的存在。
更新日期:2020-09-01
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