In this paper, a matrix‐assisted laser desorption/ionization (MALDI) Fourier transform ion cyclotron resonance (FTICR) mass spectrometry (MS) (MALDI‐FTICR‐MS) imaging method was developed to rapid and in situ detect the spatial distribution of lysophospholipids (LPLs) in zebrafish. The combination of MALDI with ultrahigh‐resolution FTICR‐MS achieves the MS imaging of LPLs with a mass resolution up to 50 000, which allows accurate identification and clear spatial visualization of LPLs in complex biological tissues. A series of lysophosphatidylcholines (LPCs) was detected using positive ion detection mode, and their concentration differences and spatial distributions were clearly visualized in different parts of zebrafish tissue. The method is rapid, simple, and efficient, being a desirable way to understand the spatial distribution of LPLs in biosome.

中文翻译：

---

使用基质辅助激光解吸/电离傅里叶变换离子回旋共振质谱对斑马鱼中的溶血磷脂进行原位检测和成像。

在本文中，开发了一种基质辅助激光解吸/电离 (MALDI) 傅里叶变换离子回旋共振 (FTICR) 质谱 (MALDI-FTICR-MS) 成像方法来快速原位检测溶血磷脂的空间分布。 (LPL) 在斑马鱼中。MALDI 与超高分辨率 FTICR-MS 相结合，实现了 LPLs 的 MS 成像，质量分辨率高达 50 000，可以准确识别复杂生物组织中的 LPLs 并实现清晰的空间可视化。使用正离子检测模式检测了一系列溶血磷脂酰胆碱（LPCs），在斑马鱼组织的不同部位清晰地显示了它们的浓度差异和空间分布。该方法快速、简便、高效，