HPLC analysis, cytotoxicity, and safety study of Moringa oleifera Lam. (wild type) leaf extract.,Journal of Food Biochemistry

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HPLC analysis, cytotoxicity, and safety study of Moringa oleifera Lam. (wild type) leaf extract.
Journal of Food Biochemistry ( IF 3.5 ) Pub Date : 2020-07-29 , DOI: 10.1111/jfbc.13400
Ammara Saleem ₁ , Mohammad Saleem _{1,

2} , Muhammad Furqan Akhtar ₃ , Mirza Muhammad Faran Ashraf Baig ₄ , Azhar Rasul ₅

Affiliation

The study was planned to evaluate toxicity of M. oleifera leaf methanol extract in Wistar rats, cytotoxic potential and chemically characterize it. Acute toxicity study revealed no mortality at 2,000 mg/kg dose. In subchronic toxicity, 150, 300, and 600 mg/kg extract were administered in both sexes for 90 days. A decrease in body weight, cholesterol, and low‐density lipoproteins, as well as an increase in the platelet count were observed. The histology of heart, lung, and kidney was normal. The oxidative stress biomarkers were normal in the liver tissue. In vitro cytotoxicity assay revealed that IC50 of the plant extract was more than 1,000 µg/ml. FTIR explored various functional groups and HPLC analysis indicated the presence of kaempferol and quercetin in the plant extract. These results showed that the plant extract might be safe up to 2,000 mg/kg single dose. The long term use of the plant extract was mostly devoid of major system toxicities.

中文翻译：

辣木的 HPLC 分析、细胞毒性和安全性研究。（野生型）叶提取物。

该研究计划评估油橄榄叶甲醇提取物对 Wistar 大鼠的毒性、细胞毒性潜力并对其进行化学表征。急性毒性研究显示，2,000 mg/kg 剂量下没有死亡。在亚慢性毒性方面，男女均服用 150、300 和 600 mg/kg 提取物，持续 90 天。观察到体重、胆固醇和低密度脂蛋白下降，以及血小板计数增加。心脏、肺和肾的组织学正常。肝组织中氧化应激生物标志物正常。体外细胞毒性测定显示植物提取物的IC50超过1,000 µg/ml。 FTIR 探索了各种官能团，HPLC 分析表明植物提取物中存在山奈酚和槲皮素。这些结果表明，单剂量 2,000 毫克/千克的植物提取物可能是安全的。植物提取物的长期使用基本上没有主要的系统毒性。

更新日期：2020-07-29

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