This work aimed to apply ultraviolet radiation as an alternative treatment to inhibit microbial growth in diesel fuel. Samples of fuel were placed in a reactor equipped with ultraviolet C (UV‐C) radiation lamps (40 W, 253 nm) and a fuel recirculation system. Assays were performed using different UV‐C exposure times (0–360 min), volume (40 and 60 L), and with / without a circulation system. The microorganisms present in diesel were quantified using the drop‐plate technique and spectrophotometric detection (600 nm), and were identified by genetic sequencing. Parameters such as color, specific mass, and kinematic viscosity were evaluated to verify whether UV radiation promotes changes in phyco‐chemical fuel properties. The assay with 360 min of radiation exposure, with a circulation system, caused the microorganism concentration to decrease from 1.50 × 10¹⁰ to 3.33 × 10² CFU mL⁻¹, but otherwise did not lead to any significant changes in fuel physicochemical properties. Microorganisms of the genera Staphylococcus sp., Enterococcus sp., Clostridium sp., Enterobacter sp. and Bacillus sp. were identified. It could be concluded that the use of UV radiation as a microbiological control method resulted in a reduction of around 99% in the concentration of the microorganisms. © 2020 Society of Chemical Industry and John Wiley & Sons, Ltd

中文翻译：

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紫外线对巴西柴油中存在的微生物失活的影响

这项工作旨在将紫外线作为替代疗法来抑制柴油中微生物的生长。燃料样品被放置在装有紫外线C（UV-C）辐射灯（40 W，253 nm）和燃料再循环系统的反应器中。使用不同的UV-C暴露时间（0–360分钟），体积（40和60 L）以及有/无循环系统进行测定。使用滴板技术和分光光度法检测（600 nm）对柴油中存在的微生物进行定量，并通过基因测序进行鉴定。对颜色，比重和运动粘度等参数进行了评估，以验证紫外线是否促进了植物化学燃料性质的变化。在360分钟的辐射照射下，通过循环系统进行的测定导致微生物浓度从1。¹⁰至3.33×10 ² CFU mL ^-1，但其他方面并未导致燃料理化性质发生任何重大变化。葡萄球菌属，肠球菌属，梭菌属，肠杆菌属的微生物。和芽孢杆菌属。被确定。可以得出结论，使用紫外线辐射作为微生物控制方法可导致微生物浓度降低约99％。©2020年化学工业协会和John Wiley＆Sons，Ltd