Influence of Cell Membrane Wrapping on the Cell-Porous Silicon Nanoparticle Interactions.,Advanced Healthcare Materials

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Influence of Cell Membrane Wrapping on the Cell-Porous Silicon Nanoparticle Interactions.
Advanced Healthcare Materials ( IF 10.0 ) Pub Date : 2020-07-29 , DOI: 10.1002/adhm.202000529
Flavia Fontana ₁ , Hanna Lindstedt ₁ , Alexandra Correia ₁ , Jacopo Chiaro ₂ , Otto K Kari ₂ , Joseph Ndika ₃ , Harri Alenius _{3,

4} , Jonas Buck ₅ , Sandro Sieber ₅ , Ermei Mäkilä ₆ , Jarno Salonen ₆ , Arto Urtti ₂ , Vincenzo Cerullo _{2,

7,

8} , Jouni T Hirvonen ₁ , Hélder A Santos _{1,

8}

Affiliation

Biohybrid nanosystems represent the cutting‐edge research in biofunctionalization of micro‐ and nano‐systems. Their physicochemical properties bring along advantages in the circulation time, camouflaging from the phagocytes, and novel antigens. This is partially a result of the qualitative differences in the protein corona, and the preferential targeting and uptake in homologous cells. However, the effect of the cell membrane on the cellular endocytosis mechanisms and time has not been fully evaluated yet. Here, the effect is assessed by quantitative flow cytometry analysis on the endocytosis of hydrophilic, negatively charged porous silicon nanoparticles and on their membrane‐coated counterparts, in the presence of chemical inhibitors of different uptake pathways. Principal component analysis is used to analyze all the data and extrapolate patterns to highlight the cell‐specific differences in the endocytosis mechanisms. Furthermore, the differences in the composition of static protein corona between naked and coated particles are investigated together with how these differences affect the interaction with human macrophages. Overall, the presence of the cell membrane only influences the speed and the entity of nanoparticles association with the cells, while there is no direct effect on the endocytosis pathways, composition of protein corona, or any reduction in macrophage‐mediated uptake.

中文翻译：

细胞膜包裹对细胞多孔硅纳米粒子相互作用的影响。

生物杂交纳米系统代表了微系统和纳米系统生物功能化方面的前沿研究。它们的理化特性在循环时间，吞噬细胞的伪装和新型抗原方面具有优势。部分原因是蛋白质电晕的质量差异以及同源细胞的优先靶向和摄取。然而，尚未完全评估细胞膜对细胞内吞作用机理和时间的影响。在这里，在存在不同吸收途径的化学抑制剂的情况下，通过定量流式细胞术分析评估了亲水性，带负电荷的多孔硅纳米粒子的内吞作用及其膜包被的内在作用。主成分分析用于分析所有数据和外推模式，以突出内吞机制中的细胞特异性差异。此外，还研究了裸露和涂层颗粒之间静态蛋白质电晕组成的差异，以及这些差异如何影响与人类巨噬细胞的相互作用。总体而言，细胞膜的存在仅影响纳米颗粒与细胞缔合的速度和实体，而对内吞途径，蛋白电晕的组成或巨噬细胞介导的摄取量的减少没有直接影响。我们研究了裸露和涂层颗粒之间静态蛋白质电晕成分的差异，以及这些差异如何影响与人类巨噬细胞的相互作用。总体而言，细胞膜的存在仅影响纳米颗粒与细胞缔合的速度和实体，而对内吞途径，蛋白质电晕的组成或巨噬细胞介导的摄取的任何降低均没有直接影响。我们研究了裸露和涂层颗粒之间静态蛋白质电晕成分的差异，以及这些差异如何影响与人类巨噬细胞的相互作用。总体而言，细胞膜的存在仅影响纳米颗粒与细胞缔合的速度和实体，而对内吞途径，蛋白电晕的组成或巨噬细胞介导的摄取量的减少没有直接影响。

更新日期：2020-09-10

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