Abstract Although Cameraria ohridella is a pest mining Aesculus hippocastanum leaves, has been known in Europe for 30 years, there is still no completely efficient method for control of this insect. The defence strategies of A. hippocastanum leaves against infestations by this pest have not been fully elucidated yet. The aim of the present study was to identify the defence strategy adopted by A. hippocastanum against the pest based on anatomical and histochemical analyses of leaves as well as to provide details of the morphology and behaviour of the developmental forms of C. ohridella. The study was carried out using a scanning, light, and fluorescence microscope. It was demonstrated that the response of the A. hippocastanum to C. ohridella larval infestations consisted in enhanced accumulation of phenolic compounds in tissues located at the border of mines and in the thickened layer of the leaf supporting the cocoon. Thorough analyses of the consecutive developmental stages of C. ohridella revealed new previously unknown features of larva L1 and some details of the mandible structures of feeding larvae L1-L4 and the characteristics of the cocoon structure, which is produced by larvae L5 and L6. We have found six stages in the developmental cycle of this pest in the present study.

中文翻译：

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七叶树发育过程中海马叶的变化

摘要 虽然Cameraria ohridella 是一种采食七叶树(Aesculus hippocastanum) 叶的害虫，在欧洲已知已有30 年的历史，但目前还没有完全有效的方法来防治这种害虫。A. hippocastanum 叶子抵御这种害虫侵袭的防御策略尚未完全阐明。本研究的目的是根据叶子的解剖和组织化学分析确定 A. hippocastanum 对害虫采取的防御策略，并提供 C. ohridella 发育形式的形态和行为的详细信息。该研究使用扫描、光学和荧光显微镜进行。结果表明，A. hippocastanum 对 C. ohridella 幼虫的侵染包括酚类化合物在位于矿井边缘的组织和支撑茧的叶子的增厚层中积累增加。对 C. ohridella 连续发育阶段的彻底分析揭示了幼虫 L1 的新的以前未知的特征和喂养幼虫 L1-L4 的下颌结构的一些细节以及由幼虫 L5 和 L6 产生的茧结构的特征。在本研究中，我们发现了这种害虫发育周期的六个阶段。ohridella 揭示了幼虫 L1 的新的以前未知的特征和喂养幼虫 L1-L4 的下颌结构的一些细节以及由幼虫 L5 和 L6 产生的茧结构的特征。在本研究中，我们发现了这种害虫发育周期的六个阶段。ohridella 揭示了幼虫 L1 的新的以前未知的特征和喂养幼虫 L1-L4 的下颌结构的一些细节以及由幼虫 L5 和 L6 产生的茧结构的特征。在本研究中，我们发现了这种害虫发育周期的六个阶段。