A high-efficiency enrichment method is required for determination of trace-level volatile terpenes in fish tissue, since the presence of such compounds in fish at elevated levels may induce bad sensory acceptance of fish meat, thus degrading its customer acceptance and consequently, its market value. In this study, a solid-phase microextraction (SPME) arrow configuration using a thick sorbent coating (120 μm, PDMS/CWR) was applied to enrich selected terpenes, namely α-pinene, limonene, linalool, and citronellol, in fish tissue (Oreochromis niloticus). Due to the thicker coating of the SPME arrow, a longer extraction time of 60 min was required to reach equilibrium extraction in comparison to the traditional fiber configuration. SPME conditions such as extraction temperature (60 °C), desorption temperature (250 °C), and salt effect (10% NaCl) were optimized for the developed application using the arrow configuration. The developed method exhibited good linearity at a concentration range of 5.0–500.0 μg L⁻¹ for α-pinene and limonene, and 50.0–500.0 μg L⁻¹ for linalool and citronellol. In addition, the coefficients of determination (R²) for all terpenes ranged from 0.9990 to 0.9999. The developed method was shown to be robust with good inter-day reproducibility in the range of 3.6–8.3%. Method sensitivity was assessed in terms of limits of detection (LODs) and limits of quantification (LOQs), with higher sensitivity achieved for α-pinene and limonene (LODs of 1.7 μg L⁻¹) in comparison to linalool (LOD, 5.0 μg L⁻¹) and citronellol (LOD, 17.0 μg L⁻¹). Theoretical calculations verified that the increased coating thickness afforded by the arrow configuration can enable higher method sensitivity and widen the range of detected compounds for the headspace SPME.

需要一种高效的富集方法来测定鱼组织中的痕量挥发性萜烯，因为此类化合物在鱼类中的含量较高可能会导致鱼肉的不良感官接受度，从而降低其对顾客的接受度，从而降低其市场占有率。值。在这项研究中，使用厚吸附剂涂层（120μm，PDMS / CWR）的固相微萃取（SPME）箭头配置应用于鱼组织中富集选定的萜烯，即α-pine烯，li烯，芳樟醇和香茅醇（尼罗罗非鱼）。由于SPME箭头的涂层较厚，与传统的纤维配置相比，达到平衡萃取需要60分钟的较长萃取时间。对于使用箭头配置的已开发应用程序，对SPME条件（例如萃取温度（60°C），解吸温度（250°C）和盐效应（10％NaCl））进行了优化。所提出的方法在5.0-500.0微克L的浓度范围内显示出良好的线性^-1为α蒎烯和苎烯，和50.0-500.0微克大号^-1为芳樟醇和香茅醇。另外，确定系数（R ²），则所有萜烯的范围从0.9990至0.9999。所开发的方法被证明是可靠的，并且具有良好的日间重现性，范围在3.6–8.3％之间。根据检测限（LOD）和定量限（LOQ）评估了方法的灵敏度，与芳樟醇（LOD，5.0μgL）相比，α-pine烯和li烯的灵敏度更高（LOD为1.7μgL ^-1）。^-1）和香茅醇（LOD，17.0μgL ^-1）。理论计算证明，由箭头配置提供的增加的涂层厚度可以实现更高的方法灵敏度，并拓宽顶空SPME的检测化合物范围。