Cryopreservation of ovarian tissues (OTs) has become the most effective way to preserve the fertility of female cancer patients. However, cryopreservation of OTs is still relatively at an experimental stage. The aim of study is to examine the effect of melatonin (MTL) on cryopreserved-thawed OTs. Fragments of OTs were cryopreserved in medium containing different concentrations (0 mM, 0.001 mM, 0.01 mM, 0.1 mM and 1 mM) of MLT. The endogenous enzymes (GSH-PX, GSH, SOD, CAT and T-AOC), MDA and ROS levels were all evaluated after cryopreservation. Our results showed that the 0.1 mM of MLT significantly improved the survival and diameter of follicles (P < 0.001). Meanwhile, the antioxidant enzymes activities (including GSH-PX, GSH, SOD, CAT and T-AOC) were enhanced and MDA content were significantly decreased in 0.1 mM of MLT group compared to other groups (P < 0.001). Additionally, compared to the control group, MTL of 0.1 mM resulted in a significantly lower ROS level. In conclusion, MLT protects the quality of cryopreserved OTs by decreasing oxidative stress level and the optimal concentration is 0.1 mM.

中文翻译：

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褪黑激素对小鼠冷冻保存卵巢组织的抗氧化作用

卵巢组织（OTs）的冷冻保存已成为保持女性癌症患者生育能力的最有效方法。然而，OTs的冷冻保存仍处于相对实验阶段。研究的目的是检查褪黑激素 (MTL) 对冻存解冻 OT 的影响。OT 的片段被冷冻保存在含有不同浓度（0 mM、0.001 mM、0.01 mM、0.1 mM 和 1 mM）的 MLT 的培养基中。内源性酶（GSH-PX、GSH、SOD、CAT 和 T-AOC）、MDA 和 ROS 水平均在冷冻保存后进行评估。我们的结果表明，0.1 mM MLT 显着提高了卵泡的存活率和直径（P < 0.001）。同时，抗氧化酶（包括GSH-PX、GSH、SOD、CAT和T-AOC）活性增强，MDA含量在0. 与其他组相比，1 mM MLT 组 (P < 0.001)。此外，与对照组相比，0.1 mM 的 MTL 导致 ROS 水平显着降低。总之，MLT 通过降低氧化应激水平来保护冷冻保存的 OTs 的质量，最佳浓度为 0.1 mM。