The movement of cell-bound membrane vesicles (CBMVs) on migrating cells is poorly understood. We hypothesized that the movement of CBMVs on migrating cells is different from that on non-migrating cells and can be interfered by external stimuli. To test it, single-vesicle tracking was performed to analyze motion type, speed, displacement, and direction of CBMVs on migrating cells treated with different reagents (Ang-1, TNF-α, LPS, VEGFα, endostatin, Cytochalasin D, and nocodazole) among which the former four promoted cell migration whereas the others inhibited cell migration. We found that cell migration changed CBMVs from non-directed to directed motion and that most CBMVs on untreated migrating cells moved along the migration axis. Interestingly, the migration-promoting reagents played positive roles in CBMV movement (improving directed motion, speed and/or maximal displacement, upregulating the amount of vesicles moving in migration direction) whereas the migration-inhibiting reagents played negative roles (impairing/abolishing directed motion, speed and/or maximal displacement, downregulating the vesicles moving forward or causing an even distribution of motion direction). The cytoskeleton (particularly microtubules) probably played vital roles in CBMV movement on migrating cells and mediated the effects of stimuli on vesicle movement. The data may provide important information for understanding the properties, behaviors, and functions of CBMVs.

人们对迁移细胞上的细胞结合膜囊泡（CBMV）的运动知之甚少。我们假设CBMVs在迁移细胞上的运动不同于在非迁移细胞上的运动，并且可能受到外部刺激的干扰。为了测试它，进行了单囊泡跟踪以分析用不同试剂（Ang-1，TNF-α，LPS，VEGFα，内皮抑素，细胞松弛素D和诺考达唑）处理的迁移细胞上CBMV的运动类型，速度，位移和方向），其中前四个促进细胞迁移，而其他四个则抑制细胞迁移。我们发现细胞迁移将CBMV从非定向运动更改为定向运动，并且未经处理的迁移细胞上的大多数CBMV沿迁移轴移动。有趣的是，迁移促进剂在CBMV运动中发挥了积极作用（改善了定向运动，速度和/或最大位移，上调了在迁移方向上移动的囊泡的数量），而抑制迁移的试剂起着负面作用（削弱/消除了定向运动，速度和/或最大位移，下调了前进的囊泡或导致了均匀的分布）运动方向）。细胞骨架（尤其是微管）可能在迁移细胞的CBMV运动中起着至关重要的作用，并介导了刺激对囊泡运动的影响。数据可为了解CBMV的特性，行为和功能提供重要信息。下调前进的囊泡或导致运动方向均匀分布）。细胞骨架（尤其是微管）可能在迁移细胞的CBMV运动中起着至关重要的作用，并介导了刺激对囊泡运动的影响。数据可为了解CBMV的特性，行为和功能提供重要信息。下调前进的囊泡或导致运动方向均匀分布）。细胞骨架（尤其是微管）可能在迁移细胞的CBMV运动中起着至关重要的作用，并介导了刺激对囊泡运动的影响。数据可为了解CBMV的特性，行为和功能提供重要信息。