Pharmaceutical compounds end up in wastewater treatment plants but little is known on their effect towards the different microbial groups in anaerobic communities. In this work, the effect of the antibiotic Ciprofloxacin (CIP), the non-steroidal anti-inflammatory drugs Diclofenac (DCF) and Ibuprofen (IBP), and the hormone 17α-ethinylestradiol (EE2), on the activity of acetogens and methanogens in anaerobic communities, was investigated. Microbial communities were more affected by CIP, followed by EE2, DCF and IBP, but the response of the different microbial groups was dissimilar. For concentrations of 0.01 to 0.1 mg/L, the specific methanogenic activity was not affected. Acetogenic bacteria were sensitive to CIP concentrations above 1 mg/L, while DCF and EE2 toxicity was only detected for concentrations higher than 10 mg/L, and IBP had no effect in all concentrations tested. Acetoclastic methanogens showed higher sensitivity to the presence of these micropollutants, being affect by all the tested pharmaceutical compounds although at different degrees. Hydrogenotrophic methanogens were not affected by any concentration, indicating their lower sensitivity to these compounds when compared to acetoclasts and acetogens.

药物化合物最终进入废水处理厂，但对厌氧菌群落中不同微生物群的作用知之甚少。在这项工作中，抗生素环丙沙星（CIP），非甾体类抗炎药双氯芬酸（DCF）和布洛芬（IBP）以及激素17α-乙炔雌二醇（EE2）对体内乙酸原和产甲烷菌活性的影响无氧社区，进行了调查。微生物群落受CIP的影响更大，其次是EE2，DCF和IBP，但不同微生物群的反应不同。对于0.01至0.1 mg / L的浓度，产甲烷比活性不受影响。产乙酸细菌对高于1 mg / L的CIP浓度敏感，而仅当浓度高于10 mg / L时才检测到DCF和EE2毒性，IBP在所有测试浓度下均无作用。破骨细胞产甲烷菌对这些微量污染物的存在表现出更高的敏感性，尽管程度不同，但受所有测试的药物化合物的影响。氢营养型产甲烷菌不受任何浓度的影响，表明与破骨细胞和产乙酸菌相比，它们对这些化合物的敏感性较低。