Sporotrichosis is one of the neglected tropical diseases causing subcutaneous chronic granulomatous lesion by thermally dimorphic fungi belonging to Sporothrix species. Sporothrix brasiliensis, Sporothrix mexicana and Sporothrix globosa are the common pathogenic species. In Asian countries, S. globosa constitutes nearly 99.3% of all Sporothrix species. We studied 63 cases of sporotrichosis of geographically diverse origin from India and Sporothrix isolates were characterised for its growth in different media, temperatures, ability to assimilate sugars and antifungal susceptibility profile. Molecular characterization was performed by sequencing of the calmodulin (CAL), beta tubulin (BT) and translational elongation factor 1-alpha (TEF-1α) and typing by fluorescent amplified fragment length polymorphism (FAFLP). In patients who presented with fixed (49.2%), lymphocutaneous lesions (23.8%), in 26.9% the details were not known, none had systemic dissemination. All the isolates tested were Sporothrix globosa and that could grow up to 35 °C and unable to grow at and beyond 37 °C. The assimilation of sucrose, ribitol and raffinose helps in identifying S. globosa. Sequences of CAL or BT or TEF-1α can differentiate S. globosa from other species in the complex. FAFLP results exhibited low genetic diversity. No correlation was noted between genotypes and clinical presentation, or geographic distribution. Itraconazole, terbinafine and posaconazole showed good in vitro antifungal activity against S. globosa whereas fluconazole and micafungin had no activity. S. globosa of Indian origin is relatively less pathogenic than other pathogenic Sporothrix species as it does not cause systemic dissemination and in the diagnostic laboratory, incubation of the cultures below 37 °C is essential for effective isolation.

中文翻译：

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印度不同来源球形孢子丝菌的表型和分子特征

孢子丝菌病是一种被忽视的热带疾病，由属于孢子丝菌属的热二态性真菌引起皮下慢性肉芽肿病变。巴西孢子丝菌、墨西哥孢子丝菌和球形孢子丝菌是常见的致病菌种。在亚洲国家，S. globosa 占所有孢子丝菌物种的近 99.3%。我们研究了来自印度的 63 例来自不同地域的孢子丝菌病病例，孢子丝菌分离株的特征在于其在不同培养基、温度、吸收糖分的能力和抗真菌药敏谱中的生长情况。通过对钙调蛋白 (CAL)、β 微管蛋白 (BT) 和翻译延伸因子 1-α (TEF-1α) 进行测序并通过荧光扩增片段长度多态性 (FAFLP) 进行分型来进行分子表征。在出现固定 (49.2%)、淋巴皮肤病变 (23.8%) 的患者中，26.9% 的细节未知，没有全身播散。测试的所有分离株都是球形孢子丝菌，它可以在 35°C 下生长，但不能在 37°C 及以上生长。蔗糖、核糖醇和棉子糖的同化有助于鉴定球形链球菌。CAL 或 BT 或 TEF-1α 的序列可以将 S. globosa 与复合体中的其他物种区分开来。FAFLP 结果表现出低遗传多样性。基因型与临床表现或地理分布之间没有发现相关性。伊曲康唑、特比萘芬和泊沙康唑对球状链球菌具有良好的体外抗真菌活性，而氟康唑和米卡芬净则没有活性。S。