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Compact 256-channel multi-well microelectrode array system for in vitro neuropharmacology test.
Lab on a Chip ( IF 6.1 ) Pub Date : 2020-07-29 , DOI: 10.1039/d0lc00384k Daejeong Kim 1 , Hongki Kang , Yoonkey Nam
Lab on a Chip ( IF 6.1 ) Pub Date : 2020-07-29 , DOI: 10.1039/d0lc00384k Daejeong Kim 1 , Hongki Kang , Yoonkey Nam
Affiliation
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Microelectrode arrays (MEAs) have been extensively used to measure extracellular spike activity from cultured neurons using multiple electrodes embedded in a planar glass substrate. This system has been implemented to investigate drug effects by detecting pharmacological perturbation reflected in spontaneous network activity. By configuring multiple wells in an MEA, a high-throughput electrophysiological assay has become available, speeding up drug tests. Despite its merits in acquiring massive amounts of electrophysiological data, the high cost and the bulky size of commercial multi-well MEA systems and most importantly its lack of customizability prevent potential users from fully implementing the system in drug experiments. In this work, we have developed a microelectrode array based drug testing platform by incorporating a custom-made compact 256-channel multi-well MEA in a standard microscope slide and commercial application-specific integrated circuit (ASIC) chip based recording system. We arranged 256 electrodes in 16 wells to maximize data collection from a single chip. The multi-well MEA in this work has a more compact design with reduced chip size compared to previously reported multi-well MEAs. Four synaptic modulators (NMDA, AMPA, bicuculline (BIC) and ATP) were applied to a multi-well MEA and neural spike activity was analyzed to study their neurophysiological effects on cultured neurons. Analyzing various neuropharmacological compounds has become much more accessible by utilizing commercially available digital amplifier chips and customizing a user-preferred analog-front-end interface design with additional benefits in reduced platform size and cost.
中文翻译：
紧凑的256通道多孔微电极阵列系统，用于体外神经药理学测试。
微电极阵列（MEA）已被广泛用于使用嵌入在平面玻璃基板中的多个电极来测量培养的神经元的细胞外刺突活性。通过检测自发网络活动中反映的药理扰动，已实施该系统以研究药物作用。通过在MEA中配置多个孔，高通量电生理测定方法已经可用，从而加快了药物测试的速度。尽管其具有获取大量电生理数据的优点，但是商业多孔MEA系统的成本高昂且体积庞大，最重要的是，其缺乏可定制性阻止了潜在用户在药物实验中全面实施该系统。在这项工作中 我们通过将定制的紧凑型256通道多孔MEA集成到标准显微镜载玻片和基于商业专用集成电路（ASIC）的记录系统中，开发了基于微电极阵列的药物测试平台。我们在16个孔中排列了256个电极，以最大程度地从单个芯片收集数据。与以前报道的多孔MEA相比，这项工作中的多孔MEA具有更紧凑的设计，并且减小了芯片尺寸。将四种突触调节剂（NMDA，AMPA，双小分子碱（BIC）和ATP）应用于多孔MEA，并分析神经突触活性以研究其对培养的神经元的神经生理作用。
更新日期：2020-09-15
中文翻译：
紧凑的256通道多孔微电极阵列系统，用于体外神经药理学测试。
微电极阵列（MEA）已被广泛用于使用嵌入在平面玻璃基板中的多个电极来测量培养的神经元的细胞外刺突活性。通过检测自发网络活动中反映的药理扰动，已实施该系统以研究药物作用。通过在MEA中配置多个孔，高通量电生理测定方法已经可用，从而加快了药物测试的速度。尽管其具有获取大量电生理数据的优点，但是商业多孔MEA系统的成本高昂且体积庞大，最重要的是，其缺乏可定制性阻止了潜在用户在药物实验中全面实施该系统。在这项工作中 我们通过将定制的紧凑型256通道多孔MEA集成到标准显微镜载玻片和基于商业专用集成电路（ASIC）的记录系统中，开发了基于微电极阵列的药物测试平台。我们在16个孔中排列了256个电极，以最大程度地从单个芯片收集数据。与以前报道的多孔MEA相比，这项工作中的多孔MEA具有更紧凑的设计，并且减小了芯片尺寸。将四种突触调节剂（NMDA，AMPA，双小分子碱（BIC）和ATP）应用于多孔MEA，并分析神经突触活性以研究其对培养的神经元的神经生理作用。
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