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Evaluation of a SARS-CoV-2 surrogate virus neutralization test for detection of antibody in human, canine, cat and hamster sera
medRxiv - Allergy and Immunology Pub Date : 2020-07-29 , DOI: 10.1101/2020.07.28.20163592
Joseph Sriyal Malik Peiris , Ranawaka APM Perera

Surrogate neutralization assays for SARS-CoV-2 that can be done without biosafety-level-3 containment and across multiple species are desirable. We evaluate a recently developed surrogate virus neutralization test (sVNT) in comparison to 90% plaque reduction neutralization tests (PRNT90) in human, canine, cat and hamster sera and found excellent concordance between the two assays. Using a panel of immune sera to other coronaviruses, we confirm the lack of cross reactivity in sVNT and PRNT90 assays.

中文翻译:

评估SARS-CoV-2替代病毒中和试验以检测人,犬,猫和仓鼠血清中的抗体

对于SARS-CoV-2的替代中和测定,可以在没有生物安全等级3的情况下进行,并且可以跨多个物种进行。与人,犬,猫和仓鼠血清中90%的斑块减少中和测试(PRNT90)相比,我们评估了最近开发的替代病毒中和测试(sVNT),并且发现两种测定之间具有极好的一致性。使用一组针对其他冠状病毒的免疫血清,我们确认了sVNT和PRNT90分析中缺乏交叉反应性。
更新日期:2020-07-29
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