The existence of crosstalk between quorum sensing systems limits their application in a complex environment. In this study, two completely orthogonal quorum sensing systems with self-produced autoinducers were built in one cell to enable the systems to be signal orthogonal and promoter orthogonal to each other. The systems were designed on the basis of the las system from Pseudomonas aeruginosa and the tra system from Agrobacterium tumefaciens. Both were optimized with respect to the orthogonality of signals and promoters by using a series of synthetic biology strategies and high-throughput screening. The systems were applied intracellularly, and an automatic delayed cascade circuit was successfully demonstrated, which can realize sequential gene expression without exogenous inducer. This circuit provides a new tool for biotechnological applications, such as metabolic regulation, that require sequential gene control. This cascade model expands the toolkit of synthetic biology research and indicates a high application potential of quorum sensing systems that are orthogonal to each other.

中文翻译：

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两个完全正交的群体感应系统带有自产自诱导器，可实现自动延迟级联控制。

群体感应系统之间串扰的存在限制了它们在复杂环境中的应用。在这项研究中，在一个细胞中构建了两个完全正交的群体感应系统，并带有自产自诱导剂，使系统信号正交和启动子正交。这些系统是在铜绿假单胞菌的 las 系统和根癌农杆菌的 tra 系统的基础上设计的. 通过使用一系列合成生物学策略和高通量筛选，在信号和启动子的正交性方面对两者进行了优化。该系统应用于细胞内，并成功展示了自动延迟级联电路，无需外源诱导剂即可实现基因顺序表达。该电路为需要顺序基因控制的生物技术应用（例如代谢调节）提供了一种新工具。这种级联模型扩展了合成生物学研究的工具包，并表明彼此正交的群体感应系统具有很高的应用潜力。