Gastric cancer is one of the most common types of carcinoma with a threat to global health. MicroRNA-760 (miR-760) was significantly down-regulated in the primary tumour of patients with advanced gastric cancer. However, the role of miR-760 in gastric cancer is still unclear. Herein, miR-760 was down-regulated in gastric cancer tissues. Moreover, miR-760 overexpression and knockdown were conducted in gastric cancer cells (MGC-803 and SGC-7901) in vitro. The in vitro functional assays proved that miR-760 overexpression reduced cell viability, cell cycle, migration and invasion, promoted apoptosis and suppressed MMP activity in MGC-803 cells. Conversely, miR-760 knockdown led to the opposite in SGC-7901 cells. Notably, bone marrow stromal antigen 2 (BST2) was verified as a target gene of miR-760. MiR-760 mimics down-regulated BST2 level in gastric cancer tissues and in MGC-803 cells, whereas miR-760 inhibitor up-regulated its level in SGC-7901 cells. MiR-760-regulated cell properties through reduction of BST2. In addition, miR-760 inhibited tumourigenesis in a nude mouse xenograft model in vivo. In conclusion, our results demonstrated that miR-760 exhibited a suppressive role in gastric cancer via inhibiting BST2, indicating that miR-760/BST2 axis may provide promising therapeutic target for gastric cancer.

中文翻译：

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MicroRNA-760通过下调胃癌中的BST2抑制细胞活力和迁移。

胃癌是威胁全球健康的最常见癌症之一。MicroRNA-760（miR-760）在晚期胃癌患者的原发肿瘤中显着下调。但是，miR-760在胃癌中的作用仍不清楚。在本文中，miR-760在胃癌组织中被下调。此外，在胃癌细胞（MGC-803和SGC-7901）中进行了miR-760的过表达和敲低。在体外功能测定证明，miR-760过表达降低了MGC-803细胞的细胞活力，细胞周期，迁移和侵袭，促进了细胞凋亡并抑制了MMP活性。相反，miR-760的敲低导致SGC-7901细胞的情况相反。值得注意的是，已证实骨髓基质抗原2（BST2）是miR-760的靶基因。MiR-760模仿了胃癌组织和MGC-803细胞中BST2的下调，而miR-760抑制剂却在SGC-7901细胞中上调了BST2的水平。通过减少BST2，MiR-760调节细胞特性。此外，miR-760在体内抑制裸鼠异种移植模型中的肿瘤发生。总之，我们的结果表明miR-760通过抑制BST2在胃癌中具有抑制作用，表明miR-760 / BST2轴可能为胃癌提供有希望的治疗靶点。