Mosquito-transmitted diseases pose a threat for a great portion of the world population. Chemical insecticides are the main tool for mosquito control. Heavy dependence on chemicals created several problems such as resistance development in many mosquito species, environmental effects, and human health issues. Other tools for mosquito control were developed and used in some parts of the world. Ribonucleic acid interference (RNAi) is a reverse genetic mechanism that was recently introduced as a new tool for pest control. Regarding mosquito, RNAi was used to study gene function and to discover genes that can be used as targets for control purposes. Several delivery methods are used to induce RNAi in mosquito larvae. Some methods such as injection and soaking are used routinely in RNAi research but have no application in the field. Other methods such as nanoparticles and microbes have some characteristics that make them good candidates for field application. In this report, we will focus on delivery methods for RNAi in mosquito larvae and will give examples for each method.

中文翻译：

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蚊子幼虫中RNAi的递送方法。

蚊虫传播疾病对世界很大一部分人口构成威胁。化学杀虫剂是控制蚊子的主要工具。对化学物质的严重依赖造成了一些问题，例如许多蚊子的抗药性发展，环境影响和人类健康问题。在世界某些地区开发并使用了其他灭蚊工具。核糖核酸干扰（RNAi）是一种逆向遗传机制，最近被引入为害虫防治的新工具。关于蚊子，RNAi用于研究基因功能并发现可用作控制目的靶标的基因。几种递送方法用于诱导蚊幼虫中的RNAi。RNAi研究通常使用一些方法，例如注射和浸泡，但在该领域没有应用。其他方法（例如纳米颗粒和微生物）具有一些特性，使其成为现场应用的良好候选者。在本报告中，我们将重点介绍蚊子幼虫中RNAi的递送方法，并提供每种方法的实例。