Mosquito-transmitted diseases pose a threat for a great portion of the world population. Chemical insecticides are the main tool for mosquito control. Heavy dependence on chemicals created several problems such as resistance development in many mosquito species, environmental effects, and human health issues. Other tools for mosquito control were developed and used in some parts of the world. Ribonucleic acid interference (RNAi) is a reverse genetic mechanism that was recently introduced as a new tool for pest control. Regarding mosquito, RNAi was used to study gene function and to discover genes that can be used as targets for control purposes. Several delivery methods are used to induce RNAi in mosquito larvae. Some methods such as injection and soaking are used routinely in RNAi research but have no application in the field. Other methods such as nanoparticles and microbes have some characteristics that make them good candidates for field application. In this report, we will focus on delivery methods for RNAi in mosquito larvae and will give examples for each method.

中文翻译：

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蚊子幼虫中 RNAi 的递送方法。


蚊媒疾病对世界上很大一部分人口构成威胁。化学杀虫剂是控制蚊子的主要工具。对化学品的严重依赖造成了一些问题，例如许多蚊子种类产生抗药性、环境影响和人类健康问题。世界一些地区还开发并使用了其他灭蚊工具。核糖核酸干扰（RNAi）是一种反向遗传机制，最近被引入作为害虫控制的新工具。对于蚊子，RNAi 用于研究基因功能并发现可用作控制目标的基因。有几种递送方法可用于在蚊子幼虫中诱导 RNAi。一些方法如注射和浸泡是RNAi研究中的常规方法，但尚未在该领域得到应用。其他方法（例如纳米粒子和微生物）具有一些特征，使其成为现场应用的良好候选者。在本报告中，我们将重点关注蚊子幼虫中 RNAi 的传递方法，并将给出每种方法的示例。