Abstract

Lecithin is a natural mixture of phospholipids and neutral lipids, and plays a vital role in the maintenance of cell-membrane integrity for all of the basic biological processes.This study aimed to evaluate the adhesion and growth of bone marrow-derived mesenchymal stem cells (BMSCs) on the three-dimensional (3D) electrospun lecithin-containing poly (L-lactic acid) (PLLA) fibrous scaffolds. In this study, PLLA and 0-15% of lecithin (wt.%) were dissolved in methylene chloride and thoroughly mixed to fabricate fibrous scaffolds by electrospinning technique. Surface morphology and hydrophilic properties of the scaffolds were characterized by scanning electron microscopy (SEM) and water uptake, respectively. The cytocompatibility of electrospun scaffolds and their potential cytotoxic effects on cells were studied over 8 days by seeding rat BMSCs on the scaffolds of PLLA containing 0-15% lecithin. Then cell proliferation was tested by CCK-8 assay and cell injury was assessed by measuring the release of intracellular lactate dehydrogenase (LDH). And cell morphology was studied by Toluidine blue staining, propidium iodide staining and SEM. The hydrophilicity was dramatically increased with an increase of lecithin content in the modified PLLA/lecithin scaffolds, as determined by rate of water uptake. In addition, the results obtained from in vitro assays suggested that the electrospun PLLA/5%lecithin scaffolds could possess the optimal hydrophilicity, higher adhesion and proliferation of BMSCs than PLLA, and they also did not lead to any significant toxic effects to cells. Moreover, BMSCs on PLLA/5%lecithin scaffolds tended to maintain their phenotypic shape and integrate with the microfibers to form a 3D cellular network, indicating a favorable interaction between this electrospun lecithin-based scaffolds and cells. The study demonstrated that the electrospun PLLA/lecithin fibrous scaffolds showed much better hydrophilicity and biocompatibility by introducing lecithin into the polymer in comparison with pure PLLA, which would offer a promising strategy for constructing tissue engnineered blood vessel.

摘要

卵磷脂是磷脂和中性脂质的天然混合物，在维持所有基本生物过程的细胞膜完整性方面起着至关重要的作用。 本研究旨在评估骨髓间充质干细胞的粘附和生长。 BMSCs）在三维（3D）电纺含卵磷脂的聚（L-乳酸）（PLLA）纤维支架上。在这项研究中，PLLA 和 0-15% 的卵磷脂 (wt.%) 溶解在二氯甲烷中，并通过静电纺丝技术充分混合以制造纤维支架。支架的表面形态和亲水性能分别通过扫描电子显微镜 (SEM) 和吸水率进行表征。通过在含有 0-15% 卵磷脂的 PLLA 支架上接种大鼠 BMSCs，研究了电纺支架的细胞相容性及其对细胞的潜在细胞毒性作用超过 8 天。然后通过CCK-8测定测试细胞增殖并通过测量细胞内乳酸脱氢酶（LDH）的释放来评估细胞损伤。并通过甲苯胺蓝染色、碘化丙啶染色和扫描电镜研究细胞形态。亲水性随着改性 PLLA/卵磷脂支架中卵磷脂含量的增加而显着增加，这由吸水率决定。此外，体外实验结果表明，与 PLLA 相比，电纺 PLLA/5% 卵磷脂支架具有最佳的亲水性，BMSCs 的粘附和增殖能力更高，而且它们也没有对细胞产生任何显着的毒性作用。此外，PLLA/5% 卵磷脂支架上的 BMSCs 倾向于保持其表型形状并与微纤维结合形成 3D 细胞网络，表明这种基于电纺卵磷脂的支架和细胞之间存在良好的相互作用。该研究表明，与纯 PLLA 相比，电纺 PLLA/卵磷脂纤维支架通过将卵磷脂引入聚合物中显示出更好的亲水性和生物相容性，这将为构建组织工程血管提供一种有前景的策略。表明这种基于电纺卵磷脂的支架和细胞之间存在良好的相互作用。该研究表明，与纯 PLLA 相比，电纺 PLLA/卵磷脂纤维支架通过将卵磷脂引入聚合物中显示出更好的亲水性和生物相容性，这将为构建组织工程血管提供一种有前景的策略。表明这种基于电纺卵磷脂的支架和细胞之间存在良好的相互作用。该研究表明，与纯 PLLA 相比，电纺 PLLA/卵磷脂纤维支架通过将卵磷脂引入聚合物中显示出更好的亲水性和生物相容性，这将为构建组织工程血管提供一种有前景的策略。