Abstract Microbial diversity, same as mineral deposits has been evaluated to be among the underground riches of a country. New data on microbial diversity are thought to be of potential economic value. Acid drainage waters are characterized by low pH and high concentrations of metals. Therefore, these acidic habitats are perfect places for quantitative and genomic-based analyses related to microbial ecology and community functions. The samplings were collected from the Kahramanmaras. The microbiological and molecular techniques were applied to determine the acidophilic community of these regions. Water samples were rich in iron (808.2 ppm, 1086.6 ppm, 2264.7 ppm), gold (1.4 ppm, 1.6 ppm, 2.5 ppm), aluminum (218.5 ppm, 265.5 ppm, 464.3 ppm), manganese (994.1 ppm, 945.6 ppm, 1643.4 ppm), sulfur (3432 ppm, 3971 ppm, 6399 ppm) and zinc (93.9 ppm, 10.8 ppm, 169.5 ppm), respectively for three seasons. The isolates were obtained as Acidiphilium sp., Acidithiobacillus sp., and Leptospirillum ferrooxidans. The predominant bacteria were found to be the members of Acidiphilium, Metallibacterium, Acidithiobacillus, Leptospirillum genera according to the results of the community fingerprint and clone library. 16S amplicon sequencing was analyzed and the diversity results were given at the genus level. Combined culture-dependent and culture-independent techniques were used to identify indigenous bacteria in the iron-rich acid mine ponds. The chemolithotrophic and heterotrophic acidophiles were isolated.

中文翻译：

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富铁环境中嗜酸细菌多样性的组合分析

摘要 微生物多样性与矿床一样，已被评价为一个国家的地下财富。微生物多样性的新数据被认为具有潜在的经济价值。酸性排水的特点是低 pH 值和高金属浓度。因此，这些酸性栖息地是与微生物生态学和群落功能相关的定量和基于基因组分析的理想场所。样本是从 Kahramanmaras 收集的。应用微生物和分子技术来确定这些区域的嗜酸群落。水样富含铁（808.2 ppm、1086.6 ppm、2264.7 ppm）、金（1.4 ppm、1.6 ppm、2.5 ppm）、铝（218.5 ppm、265.5 ppm、464.3 ppm）、锰（964.5 ppm、964.5 ppm、964.5 ppm） ppm)、硫 (3432 ppm、3971 ppm、6399 ppm) 和锌 (93.9 ppm, 10. 8 ppm、169.5 ppm），分别为三个季节。获得的分离物为嗜酸菌属、嗜酸硫杆菌属和氧化亚铁钩端螺旋体。根据群落指纹图谱和克隆文库结果，发现优势菌为嗜酸菌属、金属杆菌属、嗜酸硫杆菌属、钩端螺旋菌属。对16S扩增子测序进行分析，在属水平上给出多样性结果。结合依赖培养和不依赖培养的技术来识别富含铁的酸性矿池中的本地细菌。分离了化能嗜酸菌和异养嗜酸菌。根据群落指纹图谱和克隆文库结果，发现优势菌为嗜酸菌属、金属杆菌属、嗜酸硫杆菌属、钩端螺旋菌属。对16S扩增子测序进行分析，在属水平上给出多样性结果。结合依赖培养和不依赖培养的技术来识别富含铁的酸性矿池中的本地细菌。分离了化能嗜酸菌和异养嗜酸菌。根据群落指纹图谱和克隆文库结果，发现优势菌为嗜酸菌属、金属杆菌属、嗜酸硫杆菌属、钩端螺旋菌属。对16S扩增子测序进行分析，在属水平上给出多样性结果。结合依赖培养和不依赖培养的技术来识别富含铁的酸性矿池中的本地细菌。分离了化能嗜酸菌和异养嗜酸菌。结合依赖培养和不依赖培养的技术来识别富含铁的酸性矿池中的本地细菌。分离了化能嗜酸菌和异养嗜酸菌。结合依赖培养和不依赖培养的技术来识别富含铁的酸性矿池中的本地细菌。分离了化能嗜酸菌和异养嗜酸菌。