Abstract

New cycloartane-type triterpene 23 R/S-3β-hydroxycycloart-24-ene-23-methyl ether 1a,b (as an C-23 epimeric mixture), along with ten known compounds, including 1 steroid, 3 fatty acids and 6 triterpenoids were isolated from the aerial parts of Euphorbia dendroides L. (Euphorbiaceae). The known compounds (2–11) were identified using 1 D & 2 D-NMR spectra and by comparison with data reported in the literature as well as using GC-MS for the isolated fatty acids. The new compound 1 (a,b) was elucidated by comprehensive 1 D & 2 D NMR experiments as well as LR & HR-FAB-MS. In addition, the isolated cycloartane-type triterpenoids have been tested for in vitro cytotoxicity against different cell lines. The new compound 1 (a,b) exhibited good to weak selective cytotoxic activities against HepG2, Huh-7, KLM-1, 1321N1 and HeLa cell lines with IC₅₀ values of 20.67 ± 0.72, 16.24 ± 0.53, 22.59 ± 0.94, 25.99 ± 0.31 and 40.50 ± 3.14 μM, respectively.

摘要

新的环阿坦型三萜 23  R / S -3 β -hydroxycycloart-24-ene-23-methyl ether 1a,b（作为 C-23 差向异构体混合物），以及十种已知化合物，包括 1 种类固醇、3 种脂肪酸和从大戟科(Euphorbiaceae)地上部分分离出6种三萜类化合物。已知化合物 ( 2 – 11 ) 使用 1 D 和 2 D-NMR 光谱并通过与文献报道的数据进行比较以及使用 GC-MS 对分离的脂肪酸进行鉴定。新化合物1 (a,b)通过全面的一维和二维核磁共振实验以及 LR 和 HR-FAB-MS 进行了阐明。此外，已对分离的环阿坦型三萜类化合物进行了针对不同细胞系的体外细胞毒性测试。新化合物1 (a,b)对 HepG2、Huh-7、KLM-1、1321N1 和 HeLa 细胞系表现出良好至弱的选择性细胞毒活性，IC ₅₀值为 20.67 ± 0.72、16.24 ± 0.53、22.59 ± 0.94、25.99 ± 0.31 和 40.50 ± 3.14 μM，分别。