Abstract

The North American nightsnakes in the genus Hypsiglena is composed of nine named and at least two unnamed species. Here, we provide the first mt-genome of H. affinis, an additional mt-genome for H. sp. nov. 1, and four additional mt-genomes from the widespread H. jani. These mtDNA genomes were sequenced using both Illumina and Ion Torrent sequencing technologies. The resulting genomes contained the expected 13 protein coding genes, 22 tRNA genes, 2 rRNA genes, and 2 control regions typical of colubroid snakes. Two of the H. jani samples had partial tRNA^Ile genes upstream of CR2 which has not been previously documented in colubroid snakes. A maximum likelihood gene-tree based on these data combined with previously published sequence data recovers a well-supported phylogeny and is in concordance with previous estimates of evolutionary relationships in this group.

摘要

北美nightsnakes属中的Hypsiglena由九个命名和至少两个未命名的物种。在这里，我们提供的第一个MT基因组H.慈竹，额外的MT-基因组H.藻。十一月 1个，以及来自广泛传播的H. jani的另外四个mt基因组。这些mtDNA基因组均使用Illumina和Ion Torrent测序技术进行了测序。产生的基因组包含预期的13个蛋白质编码基因，22个tRNA基因，2个rRNA基因和2个典型的蛇形蛇控制区。H的两个。jani样本含有部分tRNA ^IleCR2上游的基因，以前在colubroid蛇中没有记录。基于这些数据与先前发布的序列数据相结合的最大似然基因树可恢复良好的系统发育，并且与该组中进化关系的先前估计相符。