Abstract

Nowadays, the expansion of nanoparticles as antimicrobial and anticancer agents is one of the most exciting methods for cancer therapy and microbial diseases that can vanquish the problems of drug resistance. In this research, the biosynthesis of silver nanoparticles (AgNPs) was stated using Summer Savory extract. Moreover, these AgNPs by UV-Vis spectroscopy, FTIR, XRD, FESEM, EDX, PSA, and zeta potential analysis were characterized, and by antimicrobial and anti-cancerous performance was evaluated. The maximum absorption peak at 472 nm by UV-Visible spectra confirmed the formation of Ag nanoparticles. The results obtained by FT-IR determined the presence of organic compounds such as amines, alkenes, alcohols, phenols, which performed significant roles in the fixating of these nanoparticles. The results obtained from FESEM, EDX, PSA, and Zeta Potential Analysis revealed that synthesized AgNPs had a hexagonal structure with a size ranging from 10 to 100 nm. The antimicrobial properties of the synthesized AgNPs were examined by Minimum Inhibitory Concentration, Minimum Bactericidal Concentration, and Minimum Fungal Concentration against five microbial strains included Klebsiella pneumonia, Escherichia coli, Enterococcus faecalis, Staphylococcus aureus, and Saccharomyces cerevisiae. The results demonstrated that biosynthesized AgNPs had the most antimicrobial activity, especially against E. Coli. The anti-cancerous results of these nanoparticles through MTT assay demonstrated exceptional anticancer effects against K-562 and MCF-7 human cancer cell lines with IC50 values 50 and 200 µg/ml, respectively.

摘要

如今，纳米颗粒作为抗微生物剂和抗癌剂的扩展已成为治疗癌症和微生物疾病的最令人振奋的方法之一，可以解决耐药性问题。在这项研究中，使用Summer Savory提取物描述了银纳米颗粒（AgNPs）的生物合成。此外，通过紫外-可见光谱，FTIR，XRD，FESEM，EDX，PSA和zeta电位分析对这些AgNP进行了表征，并通过抗菌和抗癌性能进行了评估。通过UV-可见光谱在472nm处的最大吸收峰证实了Ag纳米颗粒的形成。通过FT-IR获得的结果确定了有机化合物例如胺，烯烃，醇，酚的存在，它们在固定这些纳米颗粒中起着重要作用。从FESEM，EDX，PSA，Zeta电位分析表明，合成的AgNP具有六边形结构，尺寸为10至100nm。通过最小抑制浓度，最小杀菌浓度和最小真菌浓度对合成的AgNPs的抗菌性能进行了检测，其中包括五个菌株肺炎克雷伯菌，大肠杆菌，粪肠球菌，金黄色葡萄球菌和酿酒酵母。结果表明，生物合成的AgNP具有最大的抗菌活性，尤其是对大肠杆菌。这些纳米颗粒通过MTT分析的抗癌结果表明，它们对K-562和MCF-7人癌细胞系具有优异的抗癌作用，IC50分别为50和200 µg / ml。