ABSTRACT Diabetes mellitus is a metabolic disorder disease. Diabetes models can be established using diet and/or chemical agents. However, the effects of these methods on the intestinal microbiota are poorly understood. In this study, C57BL/6 mice were fed with a high-fat or high-sucrose-fat diet (HFD/HSFD), followed by 1 or 2 injections of streptozotocin (STZ) to induce type 2 diabetes mellitus. HFD-fed mice showed more severe dyslipidemia compared to HSFD-fed mice. The abundance of Bacteroidetes decreased in HFD-fed mice compared to HSFD-fed mice. After STZ injection, all the mice developed more severe hyperglycaemia, particularly in HFD-fed mice. Although the compositions of intestinal microbiota between the diet- and STZ-induced mice were similar, STZ injection increased the abundance of Ruminiclostridium and [Eubacterium] oxidoreducens and decreased the abundance of PrevotellaceaeUCG_001. In summary, HFD and HSFD had different metabolic phenotypes and gut microbiota. Diets play a more vital role than STZ injection in reshaping gut microbiota.

中文翻译：

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饮食和链脲佐菌素对 2 型糖尿病小鼠模型代谢和肠道微生物群的影响

【摘要】：糖尿病是一种代谢紊乱疾病。可以使用饮食和/或化学试剂建立糖尿病模型。然而，这些方法对肠道微生物群的影响知之甚少。在这项研究中，给 C57BL/6 小鼠喂食高脂肪或高蔗糖脂肪饮食 (HFD/HSFD)，然后注射 1 或 2 次链脲佐菌素 (STZ) 以诱导 2 型糖尿病。与 HSFD 喂养的小鼠相比，HFD 喂养的小鼠表现出更严重的血脂异常。与 HSFD 喂养的小鼠相比，HFD 喂养的小鼠中拟杆菌的丰度降低。注射 STZ 后，所有小鼠都出现了更严重的高血糖症，尤其是在喂食 HFD 的小鼠中。尽管饮食诱导和 STZ 诱导的小鼠肠道微生物群的组成相似，STZ注射增加了瘤胃梭菌和[真杆菌]氧化还原菌的丰度，并降低了普氏菌UCG_001的丰度。总之，HFD 和 HSFD 具有不同的代谢表型和肠道微生物群。在重塑肠道微生物群方面，饮食比注射 STZ 发挥更重要的作用。