ABSTRACT In this study, a sensitive and specific monoclonal antibody against VIR M1 was produced based on a new hapten. The antibody had a linear detection range of 3.77–65.51 ng/mL with an IC50 of 15.26 ng/mL and good specificity for VIR M1. Gold nanoparticle (GNP)-based lateral flow strips were assembled for rapid screening of VIR M1 in feed samples. When evaluated with the naked eye, the visual limit of detection (LOD) in feed samples was 25 μg/kg, and the cut-off value was 200 μg/kg. Therefore, this GNP-based lateral flow strip assay can be used for on-site detection and rapid preliminary screening of VIR M1 in feed samples.

中文翻译：

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基于金纳米颗粒的侧流条可快速灵敏地检测弗吉尼亚霉素 M1

摘要 在这项研究中，基于一种新的半抗原产生了一种针对 VIR M1 的敏感和特异的单克隆抗体。该抗体的线性检测范围为 3.77–65.51 ng/mL，IC50 为 15.26 ng/mL，对 VIR M1 具有良好的特异性。组装了基于金纳米颗粒 (GNP) 的侧流条，用于快速筛选饲料样品中的 VIR M1。用肉眼评估时，饲料样品的视觉检测限 (LOD) 为 25 μg/kg，截断值为 200 μg/kg。因此，这种基于 GNP 的侧向流条检测可用于现场检测和快速初步筛选饲料样品中的 VIR M1。