Abstract

Human serum albumin (HSA) is a biological macromolecule with important physiological functions; abnormal HSA levels are associated with coronary heart disease, multiple myeloma, diabetes, nephropathy, neurometabolic disorders, liver cirrhosis and other diseases. Therefore, accurate and quantitative detection of HAS have extremely important research and application value in biological science, molecular biology, clinical medicine and other fields. As for the detection method of HSA, dye-binding method and immune method are the first to be used, and have been applied in clinical detection. In recent years, many new detection technologies have emerged, such as fluorescent probe detection method, nano-materials for HSA detection, biosensor and so on. Although there are many methods developed recently to detect HSA, comprehensive reviews for HSA detection methods are still rare. Thus, writing this review to fill in the blank is in need. In order to highlight the recent progress in the field of HSA detection, in this review, the methods used to detect HSA are summarized and sorted, the advantages and disadvantages of these detection methods are also listed, then the research progress of small molecular fluorescence probe method is emphatically introduced in this paper. Then, we briefly discussed the challenges and future development directions in this field.

摘要

人血清白蛋白（HSA）是一种具有重要生理功能的生物大分子；HSA水平异常与冠心病、多发性骨髓瘤、糖尿病、肾病、神经代谢紊乱、肝硬化等疾病有关。因此，准确定量检测HAS在生物科学、分子生物学、临床医学等领域具有极其重要的研究和应用价值。对于HSA的检测方法，染料结合法和免疫法是最先使用的，并已应用于临床检测。近年来，出现了许多新的检测技术，如荧光探针检测方法、用于HSA检测的纳米材料、生物传感器等。尽管最近开发了许多检测 HSA 的方法，对 HSA 检测方法的全面审查仍然很少见。因此，需要写这篇评论来填补空白。为突出HSA检测领域的最新进展，本文对HSA检测方法进行了总结和梳理，并列出了这些检测方法的优缺点，然后介绍了小分子荧光探针的研究进展。本文重点介绍了方法。然后，我们简要讨论了该领域面临的挑战和未来的发展方向。还列出了这些检测方法的优缺点，然后重点介绍了小分子荧光探针法的研究进展。然后，我们简要讨论了该领域面临的挑战和未来的发展方向。还列出了这些检测方法的优缺点，然后重点介绍了小分子荧光探针法的研究进展。然后，我们简要讨论了该领域面临的挑战和未来的发展方向。