The present study focused on the development of a new method to determine the lag phase of Lactobacillus in breast milk which was attained during the 1st, 3rd, and 6th month (M1, M3, and M6). The colonies’ phylogenetic analysis, derived from the 16S rRNA gene sequences, was evaluated with genus Lactobacillus pentosus and achieved a similarity value of 99%. Raman spectroscopy in optical densities of 600 nm (OD600) were used for six consecutive days to observe the changes of the cell growth rate. The values of OD600 were well fitted with the regression model. From this work, M1 was found to be the longest lag phase in 18 h, and it was 17% to 27% longer compared to M3 and M6, respectively. However, the samples of M3 and M6 showed the shortest duration in reaching 0.5 of OD600 nm (16 h) which was enhanced by 80% and 96% compared to M1, respectively. These studies will be of significance when applied in determining the bacteria growth curve and in assessing the growth behavior for the strain in human breast milk.

中文翻译：

---

分光光度计法快速监测人乳中戊糖乳杆菌的潜力

本研究的重点是确定在第一，第三和第六个月（M1，M3和M6）达到的母乳中乳酸菌的滞后期的新方法的开发。用戊糖乳杆菌属评估了来源于16S rRNA基因序列的菌落的系统发育分析。并实现了99％的相似度值。连续六天使用光密度为600 nm（OD600）的拉曼光谱法观察细胞生长速率的变化。OD600的值与回归模型非常吻合。通过这项工作，发现M1是18小时内最长的滞后阶段，与M3和M6相比，分别延长了17％至27％。但是，M3和M6样品显示到达OD600 nm 0.5的最短时间（16小时），与M1相比分别增加了80％和96％。这些研究在确定细菌生长曲线和评估人乳中菌株的生长行为时将具有重要意义。