The pathogenesis of ischemia-reperfusion (I/R) injuries is based on oxidative stress caused by a sharp increase in the concentration of free radicals, reactive oxygen species (ROS) and secondary products of free radical oxidation of biological macromolecules during reperfusion. Application of exogenous antioxidants lowers the level of ROS in the affected tissues, suppresses or adjusts the course of oxidative stress, thereby substantially reducing the severity of I/R injury. We believe that the use of antioxidant enzymes may be the most promising line of effort since they possess higher efficiency than low molecular weight antioxidants. Among antioxidant enzymes, of great interest are peroxiredoxins (Prx1–6) which reduce a wide range of organic and inorganic peroxide substrates. In an animal model of bilateral I/R injury of kidneys (using histological, biochemical, and molecular biological methods) it was shown that intravenous administration of recombinant typical 2-Cys peroxiredoxins (Prx1 and Prx2) effectively reduces the severity of I/R damage, contributing to the normalization of the structural and functional state of the kidneys and an almost 2-fold increase in the survival of experimental animals. The use of recombinant Prx1 or Prx2 can be an efficient approach for the prevention and treatment of renal I/R injury.

中文翻译：

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肾缺血再灌注损伤中外源2-Cys过氧化物酶（Prx1和Prx2）的保护作用的比较研究。

缺血再灌注（I / R）损伤的发病机理是基于再灌注过程中生物大分子的自由基，活性氧（ROS）和自由基氧化的次级产物浓度急剧增加所引起的氧化应激。外源性抗氧化剂的应用降低了受影响组织中的ROS水平，抑制或调节了氧化应激的过程，从而大大降低了I / R损伤的严重性。我们认为抗氧化剂酶的使用可能是最有前途的工作，因为它们比低分子量抗氧化剂具有更高的效率。在抗氧化剂中，过氧化物酶（Prx1–6）引起了人们的极大兴趣，该酶可减少多种有机和无机过氧化物底物。在肾脏双侧I / R损伤的动物模型（使用组织学，生物化学和分子生物学方法）中，表明静脉内注射重组典型的2-Cys过氧化物酶（Prx1和Prx2）可有效降低I / R损伤的严重性有助于肾脏的结构和功能状态正常化，并使实验动物的存活率提高近2倍。重组Prx1或Prx2的使用可能是预防和治疗肾脏I / R损伤的有效方法。有助于肾脏的结构和功能状态正常化，并使实验动物的存活率提高近2倍。重组Prx1或Prx2的使用可能是预防和治疗肾脏I / R损伤的有效方法。有助于肾脏的结构和功能状态正常化，并使实验动物的存活率提高近2倍。重组Prx1或Prx2的使用可能是预防和治疗肾脏I / R损伤的有效方法。