In this paper, we report on the development of a microfluidic device, which extracts plasma from low‐volume whole blood (10 μL), and is aimed at integration with microfluidic system for micro analytical systems. The device autonomously separates blood cells and transfers the separated plasma by steady rotation. Based on the original autonomous fluidic control theory, we developed the principle of automatic timed injection of pressurizing medium into the separation chamber. This realizes the transferring of separated plasma from blood separation chamber to the other place, after separating blood cells by centrifugal force. Through the demonstration on a polydimethylsiloxane microfluidic device, we confirmed that the automatic plasma extraction was successfully implemented as designed. The efficiency of separation was evaluated on the basis of the concentrations of blood cells in samples before and after extraction, and the rate of blood cell removal was 98.5%.

中文翻译：

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通过自动触发控制的微血浆提取装置的建议

在本文中，我们报告了一种微流控设备的开发情况，该设备可从小体积全血（10μL）中提取血浆，并旨在与微流控系统集成用于微分析系统。该设备可自动分离血细胞并通过稳定旋转转移分离出的血浆。基于原始的自主流体控制理论，我们开发了将压力介质自动定时注入分离室的原理。在通过离心力分离血细胞之后，这实现了将分离的血浆从血液分离室转移到另一个地方。通过在聚二甲基硅氧烷微流体装置上的演示，我们确认自动血浆提取已按设计成功实施。