In addition to bone, the dentin‐pulp complex is also influenced by menopause, showing a decreased regenerative capacity. High levels of follicle‐stimulating hormone (FSH) during menopause could directly regulate bone metabolism. Here, the role of FSH in the odontogenic differentiation of the dentin‐pulp complex was investigated. Dental pulp stem cells (DPSCs) were isolated. CCK‐8 assays, cell apoptosis assays, Western blotting (WB), real‐time RT‐PCR, alkaline phosphatase activity assays, and Alizarin Red S staining were used to clarify the effects of FSH on the proliferation, apoptosis and odontogenic differentiation of the DPSCs. MAPK pathway‐related factors were explored by WB assays. FSH and its inhibitor were used in OVX rats combined with a direct pulp‐capping model. HE and immunohistochemistry were used to detect reparative dentin formation and related features. The results indicated that FSH significantly decreased the odontogenic differentiation of the DPSCs without affecting cell proliferation and apoptosis. Moreover, FSH significantly activated the JNK signalling pathway, and JNK inhibitor partly rescued the inhibitory effect of FSH on DPSC differentiation. In vivo, FSH treatment attenuated the dentin bridge formation and mineralization‐related protein expression in the OVX rats. Our findings indicated that FSH reduced the odontogenic capacity of the DPSCs and was involved in reparative dentinogenesis during menopause.

中文翻译：

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促卵泡激素会损害牙髓干细胞的牙源性分化。

除骨骼外，牙本质-纸浆复合物还受到更年期的影响，显示其再生能力降低。绝经期间高水平的促卵泡激素（FSH）可以直接调节骨代谢。在这里，研究了FSH在牙本质-牙髓复合体的牙源性分化中的作用。分离牙髓干细胞（DPSC）。CCK-8测定，细胞凋亡测定，Western印迹（WB），实时RT-PCR，碱性磷酸酶活性测定和茜素红S染色用于阐明FSH对鱼的增殖，凋亡和成牙分化的影响。 DPSC。通过WB分析探索了MAPK途径相关因素。FSH及其抑制剂在OVX大鼠中与直接牙髓压迫模型结合使用。HE和免疫组织化学用于检测修复性牙本质的形成和相关特征。结果表明，FSH显着降低了DPSC的牙源性分化，而没有影响细胞的增殖和凋亡。此外，FSH显着激活了JNK信号通路，JNK抑制剂部分挽救了FSH对DPSC分化的抑制作用。在体内，FSH处理可减轻OVX大鼠中牙本质桥的形成和矿化相关蛋白的表达。我们的研究结果表明，FSH降低了DPSC的成牙能力，并在更年期参与修复性牙本质的生成。FSH显着激活了JNK信号通路，JNK抑制剂部分挽救了FSH对DPSC分化的抑制作用。在体内，FSH处理可减轻OVX大鼠中牙本质桥的形成和矿化相关蛋白的表达。我们的研究结果表明，FSH降低了DPSC的成牙能力，并在更年期参与修复性牙本质的生成。FSH显着激活了JNK信号通路，JNK抑制剂部分挽救了FSH对DPSC分化的抑制作用。在体内，FSH处理可减轻OVX大鼠中牙本质桥的形成和矿化相关蛋白的表达。我们的研究结果表明，FSH降低了DPSC的成牙能力，并在更年期参与修复性牙本质的生成。