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Susceptibility and interactions between Aedes mosquitoes and Zika viruses
Insect Science ( IF 2.9 ) Pub Date : 2020-07-29 , DOI: 10.1111/1744-7917.12858 Teng-Fei Zhou 1 , Ze-Tian Lai 1 , Shuang Liu 1 , Jia-Yong Zhou 1 , Yang Liu 2 , Yang Wu 1 , Ye Xu 1 , Kun Wu 1 , Jin-Bao Gu 1 , Gong Cheng 3 , Xiao-Guang Chen 1
Insect Science ( IF 2.9 ) Pub Date : 2020-07-29 , DOI: 10.1111/1744-7917.12858 Teng-Fei Zhou 1 , Ze-Tian Lai 1 , Shuang Liu 1 , Jia-Yong Zhou 1 , Yang Liu 2 , Yang Wu 1 , Ye Xu 1 , Kun Wu 1 , Jin-Bao Gu 1 , Gong Cheng 3 , Xiao-Guang Chen 1
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Zika virus disease is caused by Zika virus infection, as transmitted by Aedes spp. mosquitoes. Many of the Zika virus strains isolated from patients display different pathogenicities toward humans. The vector mosquitoes for Zika virus are mainly of the Aedes genus, especially Aedes aegypti and Aedes albopictus. However, susceptibility and interactions between Aedes spp. mosquitoes and Zika viruses remain unclear. In this study, we chose two Zika virus strains (FSS13025 and PRVABC59) with different abilities to infect the primary vector mosquitoes Ae. aegypti and Ae. albopictus. The transcriptomes and small RNA profiles of infected and uninfected mosquitoes were comparatively analyzed, and differentially expressed genes were functionally examined using RNA interference. According to the results, the susceptibility of PRVABC59 was higher than that of FSS13025 in Aedes vector mosquitoes, and Ae. aegypti was more susceptible to Zika virus than was Ae. albopictus. For PRVABC59 infection, specific differential expression profiles correlated with Ae. aegypti and Ae. albopictus, and susceptibility was significantly affected when three targeted genes were successfully knocked down. Compared with PRVABC59, infection of Ae. albopictus with FSS13025 generated more 21-nt virus small interference RNA. It can be concluded that the susceptibility of vector Aedes spp. mosquitoes to Zika viruses varies and that the interactions between mosquitoes and Zika virus correlate with susceptibility.
中文翻译：
伊蚊与寨卡病毒之间的易感性和相互作用
寨卡病毒病是由寨卡病毒感染引起的，由伊蚊传播。蚊子。从患者身上分离出的许多寨卡病毒株对人类表现出不同的致病性。寨卡病毒的媒介蚊子主要是伊蚊属，特别是埃及伊蚊和白纹伊蚊。然而，伊蚊之间的易感性和相互作用。蚊子和寨卡病毒仍不清楚。在这项研究中，我们选择了两种具有不同能力的寨卡病毒株（FSS13025 和 PRVABC59）来感染主要媒介蚊子Ae。aegypti和Ae。白纹象. 比较分析受感染和未受感染蚊子的转录组和小 RNA 谱，并使用 RNA 干扰对差异表达的基因进行功能检查。结果表明，PRVABC59在伊蚊媒介蚊中的易感性高于FSS13025 ，而Ae。埃及伊蚊比埃及伊蚊更容易感染寨卡病毒。白线虫。对于 PRVABC59 感染，特定的差异表达谱与Ae相关。aegypti和Ae。当三个靶基因被成功敲低时，albopictus和易感性受到显着影响。与 PRVABC59 相比，Ae 的感染。白纹象用FSS13025产生更多的21-nt病毒小干扰RNA。可以得出结论，载体伊蚊的易感性。蚊子对寨卡病毒的影响各不相同，蚊子和寨卡病毒之间的相互作用与易感性相关。
更新日期：2020-07-29
中文翻译：
伊蚊与寨卡病毒之间的易感性和相互作用
寨卡病毒病是由寨卡病毒感染引起的，由伊蚊传播。蚊子。从患者身上分离出的许多寨卡病毒株对人类表现出不同的致病性。寨卡病毒的媒介蚊子主要是伊蚊属，特别是埃及伊蚊和白纹伊蚊。然而，伊蚊之间的易感性和相互作用。蚊子和寨卡病毒仍不清楚。在这项研究中，我们选择了两种具有不同能力的寨卡病毒株（FSS13025 和 PRVABC59）来感染主要媒介蚊子Ae。aegypti和Ae。白纹象. 比较分析受感染和未受感染蚊子的转录组和小 RNA 谱，并使用 RNA 干扰对差异表达的基因进行功能检查。结果表明，PRVABC59在伊蚊媒介蚊中的易感性高于FSS13025 ，而Ae。埃及伊蚊比埃及伊蚊更容易感染寨卡病毒。白线虫。对于 PRVABC59 感染，特定的差异表达谱与Ae相关。aegypti和Ae。当三个靶基因被成功敲低时，albopictus和易感性受到显着影响。与 PRVABC59 相比，Ae 的感染。白纹象用FSS13025产生更多的21-nt病毒小干扰RNA。可以得出结论，载体伊蚊的易感性。蚊子对寨卡病毒的影响各不相同，蚊子和寨卡病毒之间的相互作用与易感性相关。
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